Effect of Staphylococcus aureus and Staphylococcus epidermidis debris and supernatant on bone marrow stromal cells growth.

Aim: the goal of this study is to observe the effect of SA and SE debris and supernatant on BMSCs growth.

Methods: SA and SE were isolated and cultured from lesion materials of spondylitis patients which were determined by gram staining and biochemical tests. BMSCs were cultured and incubated for 11 days to be further sub-cultured and tripsinized for cell counting before seeded.

Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management.

Aim: to validate isolation and cultivation methods of bone marrow mesenchymal stem cells (BM-MSCs) from iliac crest, and to compare biological characteristics of BM-MSCs from different age groups for preparation of autologous stem cell therapy in cartilage defect.

Methods: patients undergoing spinal surgery were selected and grouped according to age. Iliac crest bone marrow from the patients was aspirated.