Publications by authors named "Mauricio Yamaguti"
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats.
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- Economic loss in pig breeding results from respiratory disorders caused primarily by Mycoplasma hyopneumoniae and Mycoplasma hyorhinis.
- The study involved analyzing swabs from 126 pigs with respiratory issues to recover these bacteria and assess their genetic differences using techniques like pulsed-field gel electrophoresis (PFGE) and 16S rRNA gene sequencing.
- Out of 59 bacterial isolates, most were Mycoplasma hyorhinis, revealing significant genetic diversity among different herds, particularly in PFGE profiles, while showing low variation in the 16S rRNA gene compared to the reference strain.
This study is the first to evaluate the occurrence of several Mollicutes species in Brazilian capuchin monkeys (Cebus spp.). Mollicutes were detected by culture and polymerase chain reaction (PCR) in samples of the oropharyngeal, conjuctiva, and genital mucosae of 58 monkeys.
View Article and Find Full Text PDFBackground: Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U.
View Article and Find Full Text PDFBackground: Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine.
View Article and Find Full Text PDFMycoplasma synoviae cell invasion: elucidation of the Mycoplasma pathogenesis in chicken.
Comp Immunol Microbiol Infect Dis
January 2011
Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was detected initially adhered to and subsequently inside the host cells. Between 24 and 48 h of infection, Ms was detected in the perinuclear region, and after 72 h of infection was confirmed by gentamicin invasion assay.
View Article and Find Full Text PDFA total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refúgio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L.
View Article and Find Full Text PDFIntraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing.
Comp Immunol Microbiol Infect Dis
January 2010
Article Synopsis
- Mycoplasma synoviae (MS) is a significant pathogen in poultry, leading to respiratory issues and joint inflammation, which impacts the Brazilian poultry industry economically.
- Researchers sequenced and analyzed the 16S rRNA gene of 19 MS strains to assess genetic variability across different Brazilian poultry production areas, discovering various polymorphic patterns.
- The study found that the number of polymorphic changes varied among strains and suggested that both spontaneous mutations over time and the movement of new strains due to management practices contribute to the observed genetic diversity.
Article Synopsis
- The study investigates genotypic variations of Ureaplasma diversum, a bacteria linked to reproductive issues in cattle, by analyzing isolates from both healthy and sick animals.
- Enzymatic digestion and pulsed-field gel electrophoresis (PFGE) were used as methods for analyzing the bacteria, highlighting the importance of timing and broth volume in obtaining quality samples.
- Although significant genetic diversity was observed among U. diversum isolates, the research found no clear link between the genetic differences and the health status of the cattle.