Effect of autologous transplant of peripheral blood mononuclear cells in combination with proangiogenic factors during experimental revascularization of lower limb ischemia.

Peripheral blood mononuclear cells (PBMCs) contain a cell fraction of mononuclear progenitor cells (MPCs), which own significant angiogenic potential. Autologous transplant of PBMC and/or platelet-rich plasma (PRP) promotes endothelial cells differentiation in experimental lower limb ischemia, which is considered a safe and effective strategy to support revascularization, either in animal models or clinical trials. In addition, thrombin has been proposed to enrich biological scaffolds, hence increasing MPC viability after intramuscular administration, whereas proangiogenic mediators such as vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α), inhibitor of the plasminogen activator-1 (PAI-1), and chemokine (CXCL1; GRO-α) participate in the endothelial response to ischemia, through their proangiogenic effects over endothelial cells proliferation, survival, migration, endothelial integrity maintenance, and physiologic vascular response to injury.

[Simplified nutritional evaluation system for surgical patients].

Objective: To determine a simplified method for nutritional status evaluation in surgical patients to identify patients prone to develop complications.

Methods: A prospective study was carried out to determine prognostic index of patients in whom major abdominal surgery was performed, based on nutritional assessment. We proposed a simplified and accessible method for daily evaluation of patients in any hospital even in an emergency setting.

[Manual mechanical anastomosis colorectal surgery].

Objective: To determine advantages and disadvantages of manual vs mechanical sutures in colon surgery.

Material And Methods: A retrospective study of clinical files of 84 colon surgery patients was conducted, to establish morbidity and mortality of these patients with special emphasis in length of surgical procedure and type of suture used during procedure (either manual or mechanical).

Results: Of 84 patients included in this study, manual suture was used in 70 (group 1) and mechanical suture in 14 (group 2).