Fixation of platelets for scanning and transmission electron microscopy.

A double fixation method of preparing platelet suspensions for both scanning and transmission electron microscopy is outlined. Prefixation in 0.1% glutaraldehyde allows for immediate preservation of morphologic characteristics induced by experimental procedures, but does not completely destroy platelet surface stickiness.

Microfollicular thyroid carcinoma with amyloid rich stroma, resembling the medullary carcinoma of the thyroid (MCT).

A human thyroid tumor is described which, on light microscopy, exhibited the features of medullary carcinoma of the thyroid (MCT). The cells were arranged in solid lobules or trabeculae, and the stroma was abundant and gave positive reaction for amyloid as assessed by congo red, crystal violet and thioflavin T stains. However, there was microfollicular differentiation in certain portions of the tumor even where the tumor was invasively growing.

Nodular renal blastema, nephroblastomatosis, and Wilms' tumor. Different points on the same disease spectrum?

A case of nodular renal blastema developing into nephroblastomatosis is presented. Definitions and histopathology of these entities is given, and it is suggested that they represent a developmental spectrum with natural progression to Wilms' tumor.

Intrapelvic migration of a threaded Steinmann pin in Parkinson's disease.

Intrapelvic migration of a threaded Steinmann pin complicated internal fixation of a displaced femoral neck fracture in an elderly man with untreated Parkinson's disease. Parkinsonian tremors may have contributed to this unusual complication. This case, in the light of a review of the literature, suggests that transfixation pins and wires should not be used in patients with Parkinson's disease.

Insulin therapy in patients with systemic insulin allergy.

Insulin was administered to 12 of 15 patients with systemic insulin hypersensitivity. Eight patients with a history of a systemic reaction to insulin but not receiving current therapy were skin-tested and desensitized. Four receiving insulin had temporary dose reduction followed by slow increase to therapeutic levels.

Respiratory immune response to aerosolized keyhole limpet hemocyanin in primate lungs.

Methodology for delivery of antigen to the lower respiratory tract of rhesus monkeys and the recovery of respiratory secretions (RS) by bronchial lavage has been evaluated and has been done on repeated occasions without apparent risk to animals. Rhesus immunoglobulin (Ig)G, IgM, and IgA were identified in RS by their cross-reactivity with human IgG, IgA, and IgM, and relative concentrations of rhesus IgA and IgG could be evaluated in sequential samples of RS and serum (S) by radial immunodiffusion analysis using antiserum specific for human gamma and alpha chains. Administration of keyhole limpet hemocyanin (KLH) by aerosol was compared with intravenous administration of KLH.

Enhanced protein adsorption at the solid-solution interface: dependence on surface charge.

By the use of infrared internal reflection spectroelectrochemistry, it has been possible to observe an enhanced adsorption of porcine fibrinogen onto a germanium surface at potentials more positive than -200 millivolts relative to a saturated calomel electrode. The enhanced adsorption was observed directly at the interface between the solid and the aqueous solution.