Publications by authors named "Matthias Griehl"
We have generated a novel, neuro-specific ncRNA microarray, covering 1472 ncRNA species, to investigate their expression in different mouse models for central nervous system diseases. Thereby, we analyzed ncRNA expression in two mouse models with impaired calcium channel activity, implicated in Epilepsy or Parkinson's disease, respectively, as well as in a mouse model mimicking pathophysiological aspects of Alzheimer's disease. We identified well over a hundred differentially expressed ncRNAs, either from known classes of ncRNAs, such as miRNAs or snoRNAs or which represented entirely novel ncRNA species.
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- The study explores the role of small non-coding RNAs (ncRNAs) in the differentiation of mouse embryonic stem (ES) cells into neural cells, noting that past research primarily focused on protein-coding genes and some microRNAs.
- To characterize the ncRNA transcriptome, researchers created three cDNA libraries from pluripotent ES cells, neural progenitors, and differentiated neural cells, using high-throughput sequencing to identify several novel miRNAs and small nucleolar RNAs involved in the process.
- Around half of the ncRNA sequences found were classified as intergenic or intragenic, with many displaying a size similar to miRNAs but lacking some typical features; these findings suggest that these ncRNAs may play significant roles in neural differentiation