Publications by authors named "Matthew Saidel"
Noninvasive prenatal screening is a long-established and widely used methodology to screen pregnancies for the most common prenatal chromosomal aneuploidies. Since 2017, positive result reports have typically included a positive predictive value to assist informed clinical decision-making. Positive predictive value is calculated based on an assay's sensitivity and specificity for a particular condition, and for the purpose of noninvasive prenatal screening, the aneuploidy's prevalence by maternal age, sometimes further adjusted by gestational age, are included in the calculation.
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- - A study investigated a rolling circle amplification (RCA) method using cell-free DNA for screening trisomies, revealing a problematic 1% false positive rate and variable assay performance, prompting a multi-center review.
- - Data from multiple labs showed significant improvement in the precision of results over time, with the percentage of runs exceeding standard deviation caps for multiple chromosomes decreasing sharply after reformulations in reagents and software updates.
- - The refined method achieved high detection rates (98.4%) and drastically reduced false positive rates (0.3%), making it competitive with other screening techniques while minimizing test failure after re-testing.
Background: Non-invasive prenatal testing (NIPT) using cell-free DNA (cfDNA) circulating in maternal blood provides a sensitive and specific screening technique for common fetal aneuploidies, but the high cost and workflow complexity of conventional methodologies limit its widespread implementation. A unique rolling circle amplification methodology reduces cost and complexity, providing a promising alternative for increased global accessibility as a first-tier test.
Methods: In this clinical study, 8160 pregnant women were screened on the Vanadis system for trisomies 13, 18, and 21, and positive results were compared to clinical outcomes where available.
Background: Studies suggest that migraine is often underdiagnosed and inadequately treated in the primary care setting, despite many patients relying on their primary care provider (PCP) to manage their migraine. Many women consider their women's healthcare provider to be their PCP, yet very little is known about migraine knowledge and practice patterns in the women's healthcare setting.
Objective: The objective of this study was to assess women's healthcare providers' knowledge and needs regarding migraine diagnosis and treatment.