Effects of Physiological-Scale Variation in Cations, pH, and Temperature on the Calibration of Electrochemical Aptamer-Based Sensors.

Electrochemical aptamer-based (EAB) sensors are the first technology supporting high-frequency, real-time, in vivo molecular measurements that is independent of the chemical reactivity of its targets, rendering it easily generalizable. As is true for all biosensors, however, EAB sensor performance is affected by the measurement environment, potentially reducing accuracy when this environment deviates from the conditions under which the sensor was calibrated. Here, we address this question by measuring the extent to which physiological-scale environmental fluctuations reduce the accuracy of a representative set of EAB sensors and explore the means of correcting these effects.

The National Capital Region HKIA attack response: A course of action for optimizing casualty receiving systems in the Military Health System.

The mass casualty event at the Hamid Karzai International Airport (HKIA) in the summer of 2021 marked the exit of the U.S. military from Afghanistan.

Refocusing the Military Health System to support Role 4 definitive care in future large-scale combat operations.

The last 20 years of sustained combat operations during the Global War on Terror generated significant advancements in combat casualty care. Improvements in point-of-injury care, en route care, and forward surgical care appropriately aligned with the survival, evacuation, and return to duty needs of the small-scale unconventional conflict. However, casualty numbers in large-scale combat operations have brought into focus the critical need for modernized casualty receiving and convalescence: Role 4 definitive care.

What Does the Military Health System Need to Support Future Combat Operations? Lessons From Aeromedical Evacuations From 2008 to 2020.

Introduction: Aeromedical evacuations from the past few decades have yielded massive amounts of data that may inform the Military Health System (MHS) on patient needs, specifically for understanding the inpatient and outpatient needs of evacuees. In this study, we evaluate inpatient and outpatient trends based on aeromedical evacuation data from recent conflicts. We anticipate that evacuations requiring MHS inpatient beds are primarily trauma-related and necessitate an increased need for inpatient trauma care.

Using seconds-resolved pharmacokinetic datasets to assess pharmacokinetic models encompassing time-varying physiology.

Aim: Pharmacokinetics have historically been assessed using drug concentration data obtained via blood draws and bench-top analysis. The cumbersome nature of these typically constrains studies to at most a dozen concentration measurements per dosing event. This, in turn, limits our statistical power in the detection of hours-scale, time-varying physiological processes.

A natural history study of coagulopathy in a porcine 40% total body surface area burn model reveals the time-dependent significance of functional assays.

Various studies have reported discordant results on the magnitude and direction of burn-induced coagulopathy (BIC), which has recently been associated with multiple organ dysfunction syndrome (MODS) and death. The increased mechanistic understanding of BIC is due, in part, to novel assays that have expanded the armamentarium beyond traditional tests like PT and aPTT. Still, BIC is a dynamic process, and the progression is difficult to define in the thermally-injured.

HIV-1 Matrix Protein Interactions with tRNA: Implications for Membrane Targeting.

The N-terminally myristoylated matrix (MA) domain of the HIV-1 Gag polyprotein promotes virus assembly by targeting Gag to the inner leaflet of the plasma membrane. Recent studies indicate that, prior to membrane binding, MA associates with cytoplasmic tRNAs (including tRNA), and in vitro studies of tRNA-dependent MA interactions with model membranes have led to proposals that competitive tRNA interactions contribute to membrane discrimination. We have characterized interactions between native, mutant, and unmyristylated (myr-) MA proteins and recombinant tRNA by NMR spectroscopy and isothermal titration calorimetry.

An Analysis and Summary of the Recommendations Made by the U.S. DoD 2010-2014 Recovering Warrior Task Force.

In 2010, the U.S. Congress directed the Department of Defense to establish the Recovering Warrior Task Force (RWTF) to examine the effectiveness of, and recommend improvements to, military programs and policies for the care, management, and transition of wounded, ill, and injured personnel.

CROWNWeb: the evolution of kidney data management. Part 3 of a three-part series.

The Centers for Medicare & Medicaid Services is developing a Web-based application, CROWNWeb, which is designed to facilitate the mandated entry and update of electronic data, and retrieve this data for dialysis facilities nationwide. Part III of this three-part series further explores the rationale behind CROWNWeb, discusses situations from the past where CROWNWeb could have benefited the renal community, and reviews how CROWNWeb can be used in CQI activities and to track and improve patient outcomes.

CROWNWeb: the evolution of kidney data management. 2.

The Centers for Medicare & Medicaid Services is developing a Web-based application, CROWNWeb, which is designed to update mandated electronic data entry and retrieval for dialysis facilities nationwide. Part 2 of this three-part series introduces CROWNWeb to the ESRD community. It discusses the release and impact of the recently released ESRD Conditions for Coverage, and provides an overview of the CMS CROWNWeb system, highlighting features and functionality.

CROWNWeb--the evolution of kidney data management (Part I).

The Centers for Medicare & Medicaid Services is developing a Web-based application, Consolidated Renal Operations in a Web-Enabled Network (CROWNWeb), which is designed to facilitate data entry, updating, and retrieval for dialysis facilities nationwide. Part 1 of this three-part series outlines the history of end-stage renal disease and Medicare, and covers the rapid growth of ESRD data management as well as other events that require progression to an online data collection and management system.

Selective recognition of pyrophosphate in water using a backbone modified cyclic peptide receptor.

A cyclic peptide based receptor, bearing two dipicolylamino arms complexed to zinc(II) ions, binds pyrophosphate ions with high affinity and selectivity in aqueous solution as determined using an indicator displacement assay.