Correction: Matthew et al. A Loop-Mediated Isothermal Amplification (LAMP) Assay Specific to Is Suitable for Use at Point-of-Care. 2022, , 594.

In the original publication [...

Roles of Cysteine Proteases in Biology and Pathogenesis of Parasites.

Cysteine proteases, also known as thiol proteases, are a class of nucleophilic proteolytic enzymes containing cysteine residues in the enzymatic domain. These proteases generally play a pivotal role in many biological reactions, such as catabolic functions and protein processing, in all living organisms. They specifically take part in many important biological processes, especially in the absorption of nutrients, invasion, virulence, and immune evasion of parasitic organisms from unicellular protozoa to multicellular helminths.

: A Neglected Protozoan Infection in the Oral Cavities of Humans and Dogs-A Scoping Review.

is a flagellated protozoan parasite found in the oral cavities of humans and animals and has been associated with periodontal disease, the most prevalent inflammatory disease affecting them all. Studies have shown that can cause damage to mammalian cells and secretes virulent proteins, such as cysteine. It is presently considered zoonotic.

Draft Genome Sequence of Strain Hs-4:NIH.

is a flagellated parasite that plays an important role in periodontal disease, with high prevalence worldwide. Its pathogenesis remains largely unknown, and there is very little information on its genome. Here, we present the whole-genome shotgun sequence of T.

A Loop-Mediated Isothermal Amplification (LAMP) Assay Specific to Is Suitable for Use at Point-of-Care.

is a flagellated protozoan that inhabits the human and canine oral cavity in patients with poor oral hygiene and periodontal disease. The loop-mediated isothermal amplification (LAMP) assay could provide clinicians with a quick, cheap and reliable diagnostic test used for the detection of in various settings. In this study, we aimed to develop a LAMP assay that can detect with high sensitivity and specificity.

Frankliniella occidentalis facilitate Salmonella enterica survival in the phyllosphere.

The human enteric bacterial pathogen Salmonella enterica causes approximately 1.35 million cases of food borne illnesses annually in the United States. Of these salmonellosis cases, almost half are derived from the consumption of fresh, raw produce.

Preoperative proteinuria is associated with increased rates of acute kidney injury after partial nephrectomy.

Purpose: We investigated the association between preoperative proteinuria and early postoperative renal function after robotic partial nephrectomy (RPN).

Patients And Methods: We retrospectively reviewed 1121 consecutive RPN cases at a single academic center from 2006 to 2016. Patients without pre-existing CKD (eGFR≥60 mL/min/1.

Predictors of positive surgical margins in patients undergoing partial nephrectomy: A large single-center experience.

Objective: To identify preoperative factors that predict positive surgical margins in partial nephrectomy.

Material And Methods: Using our institutional partial nephrectomy database, we investigated the patients who underwent partial nephrectomy for malignant tumors between January 2011 and December 2015. Patient, tumor, surgeon characteristics were compared by surgical margin status.

Variability in Partial Nephrectomy Outcomes: Does Your Surgeon Matter?

Background: Understanding physician-level discrepancies is increasingly a target of US healthcare reform for the delivery of quality-focused patient care.

Objective: To estimate the relative contributions of patient and surgeon characteristics to the variability in key outcomes after partial nephrectomy (PN).

Design, Setting, And Participants: Retrospective review of 1461 patients undergoing PN performed by 19 surgeons between 2011 and 2016 at a tertiary care referral center.

Robotic cold ischemia achieves comparable functional outcomes to open cold ischemia during partial nephrectomy for complex kidney tumors.

Objectives: To compare the perioperative and functional outcomes after open and robotic partial nephrectomy performed with cold ischemia.

Methods: A retrospective chart review was completed of consecutive patients who underwent partial nephrectomy with renal hypothermia between January 2011 and September 2016. The study cohort included both open (Open Cold Ischemia, OCI; n=170) and robotic (Robotic Cold Ischemia, RCI; n=31) patients with complex renal masses (R.

Comparative Efficacy of Laparoscopic Versus Robotic Adrenalectomy for Adrenal Malignancy.

Objective: To evaluate whether the technical advantages of robotic-assisted surgery over standard laparoscopy, which are well established for complex renal surgery, lead to variable surgical outcomes between laparoscopic adrenalectomy (LA) and robotic adrenalectomy (RA).

Methods: Using the National Cancer Database, we identified patients who underwent LA or RA for nonmetastatic primary adrenal malignancy from 2010 to 2013. Primary outcomes were need for open conversion, surgical margin status, and performance of regional lymphadenectomy.

Frozen Sections for Margins During Partial Nephrectomy Do Not Influence Recurrence Rates.

Introduction: Frozen sections (FS) are routinely employed to assess margin status during partial nephrectomy (PN) for clinically localized renal cell carcinoma (CLRCC); however, their oncologic benefit remains unclear. There have been no studies investigating the long-term impact of FS on local or metastatic recurrence. We wished to determine whether the utilization of FS for this purpose during PN influenced recurrence rates.

Trifecta Outcomes in Multifocal Tumors: A Comparison Between Robotic and Open Partial Nephrectomy.

Objectives: To report a comparative analysis of outcomes in patients who underwent multiple excisions for unilateral synchronous multifocal renal tumors using both open and robotic approaches.

Methods: We retrospectively reviewed 110 patients who underwent robotic and open partial nephrectomy and had multiple tumor excisions in an ipsilateral kidney. "Trifecta" was defined as negative surgical margins, no urologic complications, and a glomerular filtration rate (GFR) preservation of ≥90% at last follow-up.

Single-Port Robot-Assisted Perineal Prostatectomy and Pelvic Lymphadenectomy: Step-by-Step Technique in a Cadaveric Model.

The envelope is constantly being pushed to minimize the invasiveness of prostate cancer surgery without compromising oncologic or functional outcomes. Transperitoneal robot-assisted radical prostatectomy has certainly accomplished these goals. However, it is our intent to push the envelope even further, that is, to minimize the invasiveness of already minimally invasive surgery.

Significance of the nonneoplastic renal parenchymal findings in robotic partial nephrectomy series.

Aim: To describe the pathological characteristics of the peritumoral non-neoplastic renal parenchyma (NNRP) and to investigate their impact on long-term renal function after partial nephrectomy.

Materials And Methods: In our institutional robotic partial nephrectomy database, we identified 394 cases with pathological assessment of the NNRP and long-term postoperative renal functional follow-up. The NNRP was classified as normal (healthy renal parenchyma) or abnormal, based on the presence of arteriosclerosis, glomerulosclerosis, interstitial fibrosis, interstitial inflammation, and/or tubulopapillary hyperplasia.

Robotic Radical Left Nephrectomy With Inferior Vena Cava Level III Thrombectomy.

Introduction And Objective: We present our robotic approach and technique to manage a large left renal tumor with inferior vena cava (IVC) Level III thrombus. The superior mesenteric artery crossing the left renal vein requires robotic docking from the left, for dissection of the left kidney followed by repositioning and re-docking the robot from the right side for dissection of the right renal vein, distal and proximal IVC, and the remaining left renal vein insertion into the IVC.

Methods: We present a 53-year-old man with a 10.

Robotic Total Pelvic Exenteration With Intracorporeal Sigmoid Conduit and Colostomy: Step-by-Step Technique.

Objective: To describe our technique for robotic total pelvic exenteration with intracorporeal sigmoid conduit and colostomy using the da Vinci Si robot.

Methods: Three 8-mm robotic ports and two 12-mm laparoscopic ports are placed in a "W" configuration, approximately 2-3 cm more cephalad than for radical prostatectomy (Fig. 1).

Imaging strategy and outcome following partial nephrectomy.

Objectives: The aim of this study was to analyze the outcomes of surveillance after partial nephrectomy (PN) in a single institution and the relevance of imaging studies in detecting recurrence.

Material And Methods: Retrospective study of 830 patients who underwent PN for localized renal cell carcinoma between 2007 and 2015 at a single institution. We studied the characteristics of recurrence according to pathological and clinical features and elaborated risk groups.

Perinephric and Sinus Fat Invasion in Stage pT3a Tumors Managed by Partial Nephrectomy.

Introduction: We evaluated the influence of perinephric fat invasion (PFI) compared with sinus fat invasion (SFI) on disease-free survival (DFS) and cancer-specific survival (CSS) after partial nephrectomy (PN) for stage pT3a renal cell carcinoma (RCC).

Materials And Methods: Data were recorded from the consecutive records of patients who had undergone underwent PN for cT1-T2 RCC from 2007 to 2016. Of these patients, 143 had stage pT3a with SFI or PFI found on final pathologic examination.

Robotic radical perineal cystectomy and extended pelvic lymphadenectomy: initial investigation using a purpose-built single-port robotic system.

Objectives: To assess the feasibility of radical perineal cystoprostatectomy using the latest generation purpose-built single-port robotic surgical system.

Materials And Methods: In two male cadavers the da Vinci SP1098 Surgical System (Intuitive Surgical, Sunnyvale, CA, USA) was used to perform radical perineal cystoprostatectomy and bilateral extended pelvic lymph node dissection (ePLND). New features in this model include enhanced high-definition three-dimensional optics, improved instrument manoeuvrability, and a real-time instrument tracking and guidance system.

Predictors of surgical quality for retroperitoneal sarcoma: Volume matters.

Background And Objectives: The volume-outcome relationship is well recognized. We sought to investigate this relationship in retroperitoneal sarcoma (RPS) surgery.

Methods: Non-metastatic RPS cases from 2004 to 2014 in the National Cancer Database were analyzed.

Cytoreductive nephrectomy in the modern era: Predictors of use, morbidity, and survival.

Introduction: To determine tumour, patient, and provider factors associated with cytoreductive nephrectomy (CN) use and to identify those factors that predicted short-term and long-term surgical outcomes.

Methods: We performed a retrospective review (1998-2011) of the National Cancer Database, a U.S.

Optimum outcome achievement in partial nephrectomy for T1 renal masses: a contemporary analysis of open and robot-assisted cases.

Objectives: To compare optimum outcome achievement in open partial nephrectomy (OPN) with that in robot-assisted partial nephrectomy (RAPN).

Patients And Methods: Using our institutional partial nephrectomy (PN) database, we reviewed 605 cases performed for unifocal clinical T1 renal masses in non-solitary kidneys between 2011 and 2015. Tetrafecta, which was defined as negative surgical margins, freedom from peri-operative complications, ≥80% renal function preservation, and no chronic kidney disease upstaging, was chosen as the composite optimum outcome.

Hilar Parenchymal Oversew: a novel technique for robotic partial nephrectomy hilar tumor renorrhaphy.

Introduction: A renorrhaphy technique which is effective for hemostasis but does not place undue tension on the branch vessels of the renal sinus remains one of the challenging steps after hilar tumor resection during robotic partial nephrectomy (RPN). The published V-hilar suture (VHS) technique is one option for reconstruction after an RPN involving the hilum. The objective of this video is to show a novel renorrhaphy technique, Hilar Parenchymal Oversew that has been effective for such cases.