Publications by authors named "Matthew J Zubrowski"
Article Synopsis
- EMT is linked to tumor cell behavior like migration and metastasis, and researchers are looking for ways to reverse this process using shRNA libraries to identify kinase targets.
- No single kinase suppression was effective on its own to trigger EMT, but knocking down multiple kinases, especially MAPK7, prompted changes in epithelial markers.
- Targeting kinases like MAPK7 could reduce tumor invasiveness by promoting epithelial characteristics in cancer cells and decreasing the spread of metastatic tumors.
To define the functional pathways regulating epithelial cell migration, we performed a genome-wide RNAi screen using 55,000 pooled lentiviral shRNAs targeting ∼11,000 genes, selecting for transduced cells with increased motility. A stringent validation protocol generated a set of 31 genes representing diverse pathways whose knockdown dramatically enhances cellular migration. Some of these pathways share features of epithelial-to-mesenchymal transition (EMT), and together they implicate key regulators of transcription, cellular signaling, and metabolism, as well as novel modulators of cellular trafficking, such as DLG5.
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