Publications by authors named "Matthew J Coussens"
Article Synopsis
- - Genome editing enables researchers to understand gene functions and disease genetics more precisely than traditional methods, which often lead to random and unwanted genetic changes.
- - Zinc finger nucleases (ZFNs) are crucial for targeted genome editing, as they can cut specific DNA sequences and facilitate efficient gene modifications without needing chemicals or synthetic DNA.
- - The CompoZr Custom ZFN Service offers a systematic approach for researchers, providing support in analyzing gene structure and ensuring targeted gene editing is successful.
Article Synopsis
- The Target ID Library helps researchers identify microRNA (miRNA) targets by using a plasmid-based cDNA library that includes sequences from various human tissues and cell lines.
- It involves a multi-step selection process where stable transformants first grow, followed by the introduction of a specific miRNA, and finally selecting for cDNAs that contain the miRNA's target.
- The library covers a large portion of the human transcriptome, with over 79% of unique genes represented, and has been sequenced to confirm its diversity and effectiveness in identifying gene targets.
RNA interference (RNAi) is an intrinsic cellular mechanism for the regulation of gene expression. Harnessing the innate power of this system enables us to knockdown gene expression levels in loss of gene function studies. There are two main methods for performing RNAi.
View Article and Find Full Text PDFSIRT1, the mammalian homolog of SIR2 in Saccharomyces cerevisiae, is an NAD-dependent deacetylase implicated in regulation of lifespan. By designing effective short hairpin RNAs and a silent shRNA-resistant mutant SIRT1 in a genetically defined system, we show that efficient inhibition of SIRT1 in telomerase-immortalized human cells enhanced cell growth under normal and nutrient limiting conditions. Hematopoietic stem cells obtained from SIRT1-deficient mice also showed increased growth capacity and decreased dependency on growth factors.
View Article and Find Full Text PDFThe NRAMP 1 gene is a major candidate gene influencing the outcome of infections with intracellular pathogens in numerous species. NRAMP 1 is highly conserved in many mammalian species and the NRAMP 1 gene shows considerable conservation in structure between mice and humans. The association of NRAMP 1 gene polymorphisms with disease in cattle has been limited to a single microsatellite located within the 3'-non coding region of the bovine NRAMP 1 gene.
View Article and Find Full Text PDFA cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortium's (NBFGC) expressed sequence tag (EST) collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues.
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