Publications by authors named "Matthew H McDonough"
Electrochemical aptamer-based (EAB) sensors are the first technology supporting high-frequency, real-time, in vivo molecular measurements that is independent of the chemical reactivity of its targets, rendering it easily generalizable. As is true for all biosensors, however, EAB sensor performance is affected by the measurement environment, potentially reducing accuracy when this environment deviates from the conditions under which the sensor was calibrated. Here, we address this question by measuring the extent to which physiological-scale environmental fluctuations reduce the accuracy of a representative set of EAB sensors and explore the means of correcting these effects.
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- Traditional methods for measuring drug concentrations in the brain lack real-time capabilities and have poor temporal resolution, limiting the understanding of drug effects in behaving subjects.
- Electrochemical aptamer-based sensors have been developed that allow for real-time, seconds-resolved measurements of drug concentrations, achieving precise detection limits and enabling the study of pharmacokinetics in freely moving rats.
- The study shows that these sensors can maintain constant drug levels in the brain for extended periods, highlighting their potential for site-specific drug delivery and analysis of concentration-behavior relationships in individual subjects.
Aim: Pharmacokinetics have historically been assessed using drug concentration data obtained via blood draws and bench-top analysis. The cumbersome nature of these typically constrains studies to at most a dozen concentration measurements per dosing event. This, in turn, limits our statistical power in the detection of hours-scale, time-varying physiological processes.
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