Unraveling the influences of sequence and position on yeast uORF activity using massively parallel reporter systems and machine learning.

Upstream open-reading frames (uORFs) are potent -acting regulators of mRNA translation and nonsense-mediated decay (NMD). While both AUG- and non-AUG initiated uORFs are ubiquitous in ribosome profiling studies, few uORFs have been experimentally tested. Consequently, the relative influences of sequence, structural, and positional features on uORF activity have not been determined.