Late-stage functionalization of the 4-amino-2-pyridone chemotype using electrochemical and MCR approaches.

We report the development and application of different strategies for the late-stage functionalization (LSF) of the biologically relevant 4-amino-2-pyridone chemotype. Using the recently discovered PCSK9 inhibitor 5c as a prototype, a series of electrochemical LSF (e-LSF) and multicomponent LSF (MCR-LSF) have been set-up to decorate the 4-amino-2-pyridone scaffold. The usefulness of these methods has been demonstrated by generating a series of novel derivatives in a site-selective and sustainable way.

LAI estimation through remotely sensed NDVI following hail defoliation in maize ( L.) using Sentinel-2 and UAV imagery.

Unlabelled: Extreme events such as hailstorms are a cause for concern in agriculture, leading to both economic and food supply losses. Traditional damage estimation techniques have recently been called into question since damages have rarely been quantified at the large-field scale. Damage-estimation methods used by field inspectors are complex and sometimes subjective and hardly account for damage spatial variability.

Protein Maps for Durum Wheat Precision Harvest and Pasta Production.

The quality traits of dough and dry pasta obtained from semolina (Triticum durum Desf. var. Biensur), harvested from a 13.

Trade-offs among ecosystem services advance the case for improved spatial targeting of agri-environmental measures.

Agri-environmental measures (AEMs) are meant to foster environmentally-friendly farming techniques. The use of AEMs to enhance agroecosystem quality is still under debate due to site-specific spatial mismatches that often occur between adopted AEMs and delivered ecosystem services. Here, a site-specific approach was employed to assess the advantages and disadvantages of AEMs adopted from the Rural Development Programme and applied in the Veneto Region (NE Italy) during 2014-2020.

Changes in classic and alternative pathways of bile acid synthesis in chronic liver disease.

Background: Cholesterol elimination occurs through bile acid synthesis that starts within the liver from 7alpha-hydroxylation or in extrahepatic tissues from 27-hydroxylation. This study was aimed at investigating in vivo these two pathways in patients with chronic liver disease.

Methods: Serum concentrations of 7alpha- and 27-hydroxycholesterol were measured in 54 patients (29 with primary biliary cirrhosis and 25 with chronic hepatitis C) and 18 controls.

A minimally invasive technique for the evaluation of the regulatory steps of the two major pathways of bile acid synthesis.

Background: Bile acid synthesis accounts for more than 95% of total cholesterol catabolism per day. We have developed a minimally invasive technique in humans that quantifies the rates of plasma appearance of 7alpha- and 27-hydroxycholesterol, representing the first steps of the "classical" and "alternative" pathways of bile acid synthesis, respectively.

Methods: For this purpose, during the intravenous infusion of synthetic deuterated isotopomers of 7alpha-hydroxycholesterol and 27-hydroxycholesterol plasma samples are collected and analysed by a GC-MS based method that allows to quantify the exogenous/natural isotopomer ratio of the two sterols.

Hyperlipidemia in Chronic Cholestatic Liver Disease.

Hyperlipidemia with a marked increase of low-density lipoprotein (LDL) and high- density lipoprotein (HDL) cholesterol levels is a common feature in patients with chronic cholestatic liver disease. Excess morbidity and mortality from cardiovascular disease has not been reported in these patients. This may be due to the particular lipoprotein pattern observed during chronic cholestasis, characterized by elevated serum HDL cholesterol, which may have a cardioprotective effect.