Maximizing DNA profiling success from sub-optimal quantities of DNA: a staged approach.

Obtaining genetic profiles from samples containing minimal amounts of DNA can be difficult. In forensic science, the vast majority of genetic profiles are generated using commercial kits that have been optimized for the amplification of a specific range of DNA concentrations. DNA extracted from many forensic samples falls below the kit manufacturers' specified concentrations either because there is not enough total DNA in the extract or the extract is so dilute that not enough volume of the extract can be added to the PCR.

National recommendations of the Technical UK DNA working group on mixture interpretation for the NDNAD and for court going purposes.

The Technical UK DNA working group comprises representatives from all of the major suppliers of the UK and Ireland who contribute to the UK national DNA database. The group has the following terms of reference:To act as a peer review body.To agree experimental designs, to provide advice to the custodian to facilitate the development of the NDNAD.