Contribution of cytochrome b5 to androgen synthesis in rat testicular microsomes.

Cytochrome b5 was purified from porcine testicular microsomes, and its amino acid composition was determined. Rabbit antibody against the purified cytochrome b5 was prepared in order to study the contribution of cytochrome b5 to testicular microsomal oxygenases related to androgen production. In the presence of NADPH alone as the electron donor, the antibody against cytochrome b5 inhibited the activities of steroid 17 alpha-hydroxylase and C-17-C-20 lyase of rat testicular microsomal fraction.

Absorption, distribution and excretion of 14C-pirenzepine in rats. Accumulation characteristics after multiple dose regimen.

Absorption, distribution and excretion of 14C-labelled 5,11-dihydro-11-[(4-methyl-1-piperazinyl) acetyl]-6H-pyrido[2,3-b][1,4]-benzodiazepin-6-one-dihydrochloride (pirenzepine, LS-519 Cl2) were studied in SD-JCL rats. After a single i.v.

Comparative studies on the fate of 14C-dipyridamole (RA8) after single and multiple administration in rats.

1) Absorption, distribution and excretion of 14C-Dipyridamole (RA 8) wee studied comparatively after the administration of single intravenous (5 mg/kg), single oral (10 mg/kg) and multiple oral (10 mg/kg, once a day for one week) dosages in rats. 2) The blood level of radioactivity after oral administration showed a monophasic slow elimination with a half-life (t1/2) of about 10 hrs. The apparent absorption rate was not so high (t1/2 : 0.

Effects of fominoben-HCl on rat liver.

The effect of Fominoben-HCl (Noleptan) was studied in male rats given 200 mg/kg/day over one, two or four weeks. In all three experiments a slight enlargement of the liver was observed. After one-week treatment, activity of microsomal mixed-function oxidase was stimulated without an increase in cytochrome P-450 content.

Studies on the formation of cyclohexylamine and N-methylcyclohexylamine from bromhexine in animals and man, and simultaneous determination of cyclohexylamine and N-methylcyclohexylamine by gas chromatography.

In order to detect cyclohexylamine and N-methylcyclohexylamine simultaneously in urine, a gas chromatographic method was developed in which Chromosorb 103 (porous polymer) was found to be suitable as the column packing. The detection limits were 0.1 mug/ml of cyclohexylamine and 0.