Evaluation of 6 years of eHealth data in the alcohol use disorder field indicates improved efficacy of care.

Background: Predictive eHealth tools will change the field of medicine, however long-term data is scarce. Here, we report findings on data collected over 6 years with an AI-based eHealth system for supporting the treatment of alcohol use disorder.

Methods: Since the deployment of Previct Alcohol, structured data has been archived in a data warehouse, currently comprising 505,641 patient days.

Inertial focusing with sub-micron resolution for separation of bacteria.

In this paper, we study inertial focusing in curved channels and demonstrate the alignment of particles with diameters between 0.5 and 2.0 μm, a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells.

The Synchronization of Replication and Division Cycles in Individual E. coli Cells.

Isogenic E. coli cells growing in a constant environment display significant variability in growth rates, division sizes, and generation times. The guiding principle appears to be that each cell, during one generation, adds a size increment that is uncorrelated to its birth size.

Direct measurement of transcription factor dissociation excludes a simple operator occupancy model for gene regulation.

Transcription factors mediate gene regulation by site-specific binding to chromosomal operators. It is commonly assumed that the level of repression is determined solely by the equilibrium binding of a repressor to its operator. However, this assumption has not been possible to test in living cells.

Studying transcriptional interactions in single cells at sufficient resolution.

Our ability to dissect and understand the principles of gene regulatory circuits is partly limited by the resolution of our experimental assays. In this brief review, we discuss aspects of gene expression in microbial organisms apparent only when increasing the experimental resolution from populations to single cells and sub-cellular structures, from snap-shots to high-speed time-lapse movies and from molecular ensembles to single molecules.