Tunable entangled photon-pair generation in a liquid crystal.

Liquid crystals, with their ability to self-assemble, strong response to an electric field and integrability into complex systems, are key materials in light-beam manipulation. The recently discovered ferroelectric nematic liquid crystals also have considerable second-order optical nonlinearity, making them a potential material for nonlinear optics. Their use as sources of quantum light could considerably extend the boundaries of photonic quantum technologies.

Intracellular biocompatible hexagonal boron nitride quantum emitters as single-photon sources and barcodes.

Color centers in hexagonal boron nitride (hBN) have been emerging as a multifunctional platform for various optical applications including quantum information processing, quantum computing and imaging. Simultaneously, due to its biocompatibility and biodegradability hBN is a promising material for biomedical applications. In this work, we demonstrate single-photon emission from hBN color centers embedded inside live cells and their application to cellular barcoding.

Quantifying local stiffness and forces in soft biological tissues using droplet optical microcavities.

Mechanical properties of biological tissues fundamentally underlie various biological processes and noncontact, local, and microscopic methods can provide fundamental insights. Here, we present an approach for quantifying the local mechanical properties of biological materials at the microscale, based on measuring the spectral shifts of the optical resonances in droplet microcavities. Specifically, the developed method allows for measurements of deformations in dye-doped oil droplets embedded in soft materials or biological tissues with an error of only 1 nm, which in turn enables measurements of anisotropic stress inside tissues as small as a few pN/μm.

Non-contact monitoring of glucose concentration and pH by integration of wearable and implantable hydrogel sensors with optical coherence tomography.

Optical coherence tomography (OCT) is a noninvasive imaging technique with large penetration depth into the tissue, but limited chemical specificity. By incorporating functional co-monomers, hydrogels can be designed to respond to specific molecules and undergo reversible volume changes. In this study, we present implantable and wearable biocompatible hydrogel sensors combined with OCT to monitor their thickness change as a tool for continuous and real-time monitoring of glucose concentration and pH.

Microcavity- and Microlaser-Based Optical Barcoding: A Review of Encoding Techniques and Applications.

Optical microbarcodes have recently received a great deal of interest because of their suitability for a wide range of applications, such as multiplexed assays, cell tagging and tracking, anticounterfeiting, and product labeling. Spectral barcodes are especially promising because they are robust and have a simple readout. In addition, microcavity- and microlaser-based barcodes have very narrow spectra and therefore have the potential to generate millions of unique barcodes.

Deep tissue localization and sensing using optical microcavity probes.

Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging.

Topological liquid crystal superstructures as structured light lasers.

Liquid crystals (LCs) form an extremely rich range of self-assembled topological structures with artificially or naturally created topological defects. Some of the main applications of LCs are various optical and photonic devices, where compared to their solid-state counterparts, soft photonic systems are fundamentally different in terms of unique properties such as self-assembly, self-healing, large tunability, sensitivity to external stimuli, and biocompatibility. Here we show that complex tunable microlasers emitting structured light can be generated from self-assembled topological LC superstructures containing topological defects inserted into a thin Fabry-Pérot microcavity.

From coffee stains to uniform deposits: Significance of the contact-line mobility.

Hypothesis: Contact-line motion upon drying of a sessile droplet strongly affects the solute transport and solvent evaporation profile. Hence, it should have a strong impact on the deposit formation and might be responsible for volcano-like, dome-like and flat deposit morphologies.

Experiments: A method based on a thin-film interference was used to track the drop height profile and contact line motion during the drying.

Particulate Matter (PM) from Biomass Combustion Induces an Anti-Oxidative Response and Cancer Drug Resistance in Human Bronchial Epithelial BEAS-2B Cells.

Nearly half of the world's population relies on combustion of solid biofuels to cover fundamental energy demands. Epidemiologic data demonstrate that particularly long-term emissions adversely affect human health. However, pathological molecular mechanisms are insufficiently characterized.

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision.

Droplets with predefined sizes have been controllably produced at the tip of a micro-capillary immersed in an external fluid while tracking the high Q-factor whispering gallery modes (WGM). The modes were fitted to a model to give precise real-time size measurement, which was used as a feedback to control the pressure in the capillary and the release of the droplet from the capillary when it reached the target size. In this way a dispersion of highly monodisperse droplets anywhere in the size range from 5 μm to 50 μm were produced.

Bifunctional Duocarmycin Analogues as Inhibitors of Protein Tyrosine Kinases.

Bifunctional duocarmycin analogues are highly cytotoxic compounds that have been shown to be irreversible aldehyde dehydrogenase 1 inhibitors. Interestingly, cells with low aldehyde dehydrogenase 1 expression are also sensitive to bifunctional duocarmycin analogues, suggesting the existence of another target. Through in silico approaches, including principal component analysis, structure-similarity search, and docking calculations, protein tyrosine kinases, and especially the vascular endothelial growth factor receptor 2 (VEGFR-2), were predicted as targets of bifunctional duocarmycin analogues.

Biomaterial microlasers implantable in the cornea, skin, and blood.

Stand-alone laser particles that are implantable into biological tissues have potential to enable novel optical imaging, diagnosis and therapy. Here we demonstrate several types of biocompatible microlasers and their lasing action within biological systems. Dye-doped polystyrene beads were embedded in the cornea and optically pumped to generate narrowband emission.

Remote and autonomous temperature measurement based on 3D liquid crystal microlasers.

We demonstrate non-contact temperature measurement with one tenth of a kelvin precision at distances of several meters using omnidirectional laser emission from dye-doped cholesteric liquid crystal droplets freely floating in a fluid medium. Upon the excitation with a pulsed laser the liquid crystal droplet emits laser light due to 3D Bragg lasing in all directions. The spectral position of the lasing is highly dependent on temperature, which enables remote and contact-less temperature measurement with high precision.

Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies.

Fluorescence and bioluminescence are widely used to study biological systems from molecular to whole organism level. However, their broadband emission is often a bottleneck for sensitive spectral measurements and multiplexing. To overcome the limitation, the emitters can be coupled with optical cavity modes to generate narrowband spectral features.

Disease relevant modifications of the methylome and transcriptome by particulate matter (PM) from biomass combustion.

Exposure to particulate matter (PM) is recognized as a major health hazard, but molecular responses are still insufficiently described. We analyzed the epigenetic impact of ambient PM from biomass combustion on the methylome of primary human bronchial epithelial BEAS-2B cells using the Illumina HumanMethylation450 BeadChip. The transcriptome was determined by the Affymetrix HG-U133 Plus 2.

Spectral reading of optical resonance-encoded cells in microfluidics.

The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells.

Stress fibers, autophagy and necrosis by persistent exposure to PM2.5 from biomass combustion.

Fine particulate matter (PM2.5) can adversely affect human health. Emissions from residential energy sources have the largest impact on premature mortality globally, but their pathological and molecular implications on cellular physiology are still elusive.

Optical lens-microneedle array for percutaneous light delivery.

The limited penetration depth of light in skin tissues is a practical bottleneck in dermatologic applications of light-induced therapies, including anti-microbial blue light therapy and photodynamic skin cancer therapy. Here, we demonstrate a novel device, termed optical microneedle array (OMNA), for percutaneous light delivery. A prototype device with a 11 by 11 array of needles at a spacing of 1 mm and a length of 1.

Laser Particle Stimulated Emission Microscopy.

We introduce an optical microscopy technique that utilizes micro- or nanolasers embedded in a sample as imaging probes. The narrow spectra and nonlinear power dependence of stimulated emission from the laser particles yield optical sectioning, subdiffraction resolution, and low out-of-focus background. A proof of concept is demonstrated using perovskite nanowires.

Lasing properties of polymerized chiral nematic Bragg onion microlasers.

Dye doped photocurable cholesteric liquid crystal was used to produce solid Bragg onion omnidirectional lasers. The lasers were produced by dispersing and polymerizing chiral nematic LC with parallel surface anchoring of LC molecules at the interface, extracted and transferred into another medium. Lasing characteristics were studied in carrier medium with different refractive index.

Isolation of Flavonoids from Deguelia duckeana and Their Effect on Cellular Viability, AMPK, eEF2, eIF2 and eIF4E.

Preparations of Deguelia duckeana, known in Brazil as timbó, are used by indigenous people to kill fish. Reinvestigation of its extracts resulted in the isolation and identification of 11 known flavonoids identified as 3,5,4'-trimethoxy-4-prenylstilbene (1), 4-methoxyderricidine (2), lonchocarpine (3), 4-hydroxylonchocarpine (4), 4-methoxylonchocarpine (5), 5-hydroxy-4',7-dimethoxy-6-prenylflavanone (6), 4'-hydroxyisolonchocarpine (7), 4'-methoxyisolonchocarpine (8), 3',4',7-trimethoxyflavone (9), 3',4'-methylenedioxy-7-methoxyflavone (10), and 2,2-dimethyl-chromone-5,4'-hydroxy-5'-methoxyflavone (11). Except for 1, 3, and 4 all of these flavonoids have been described for the first time in D.

Bioabsorbable polymer optical waveguides for deep-tissue photomedicine.

Advances in photonics have stimulated significant progress in medicine, with many techniques now in routine clinical use. However, the finite depth of light penetration in tissue is a serious constraint to clinical utility. Here we show implantable light-delivery devices made of bio-derived or biocompatible, and biodegradable polymers.

Photonic hydrogel sensors.

Analyte-sensitive hydrogels that incorporate optical structures have emerged as sensing platforms for point-of-care diagnostics. The optical properties of the hydrogel sensors can be rationally designed and fabricated through self-assembly, microfabrication or laser writing. The advantages of photonic hydrogel sensors over conventional assay formats include label-free, quantitative, reusable, and continuous measurement capability that can be integrated with equipment-free text or image display.