Publications by authors named "Matilde E Fuentes"
Acta Biochim Pol
December 2006
Article Synopsis
- The study analyzes a proposed reaction scheme for zymogen activation, focusing on the roles of intra- and intermolecular routes and the enzyme-zymogen complex.
- It introduces new dimensionless parameters to help quantify the contributions of these routes over time based on rate constants and initial conditions.
- The findings are applied to the specific case of pepsinogen activation into pepsin, utilizing existing kinetic parameters from previous research.
Article Synopsis
- The text presents a mathematical model for how zymogens (inactive enzymes) activate into active enzymes through both intra- and intermolecular interactions.
- It includes a complex formation step that allows for the definition of a Michaelis-Menten constant specific to the activation process.
- The paper also outlines methods for experimental design and data analysis to assess kinetic parameters and verifies the model with simulated data, specifically using the transformation of pepsinogen to pepsin as an example.
