Strain T-12, an orange, Gram-stain-negative, non-motile, rod-shaped strain, was isolated in November 2013 from water samples collected from an Atlantic salmon () fry culturing system at a fish farm in Chile. Phylogenetic analysis based on 16S rRNA sequences (1394 bp) revealed that strain T-12 belonged to the genus , showing close relationships to F-372 (99.48 %) and DS-20 (98.
View Article and Find Full Text PDFStrain LB-N7, a novel Gram-negative, orange, translucent, gliding, rod-shaped bacterium, was isolated from water samples collected from an open system of Atlantic salmon () smolts in a fish farm in Chile during a flavobacterial infection outbreak in 2015. Phylogenetic analysis based on 16S rRNA sequences (1337 bp) revealed that strain LB-N7 belongs to the genus and is closely related to the type strains A2-1 (98.8 %) and R2A45-3 (96.
View Article and Find Full Text PDFStrain I-SCBP12n, a novel Gram-stain-negative, aerobic, non-spore-forming, motile-by-gliding and rod-shaped bacterium, was isolated from a chinstrap penguin chick () during a 2015 expedition to the Chilean Antarctic territory. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I-SCBP12n belonged to the genus , being closely related to strains P3160 (98.52 %), WB 4.
View Article and Find Full Text PDFis a phytopathogenic fungus that infects over 200 plant species and can cause significant crop losses in local and worldwide agricultural industries. However, its presence in the endemic flora in the Coquimbo Region and its impact on local flora have not been studied yet. In order to determine whether spp is present in the native plant in the Coquimbo Region, fifty-two field-samples were analysed.
View Article and Find Full Text PDFThe air humidity in Antarctica is very low and this peculiar weather parameter make the use of flame retardants in research facilities highly needed for safety reasons, as fires are a major risk. Legacy and novel flame retardants (nFRs) including polybrominated diphenyl ethers (PBDEs), hexabromocyclododecanes (HBCDs), 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), Dechlorane Plus (DP), and other nFRs were measured in indoor dust samples collected at research Stations in Antarctica: Gabriel de Castilla, Spain (GCS), Julio Escudero, Chile (JES), and onboard the RRS James Clark Ross, United Kingdom (RRS JCR). The GC-HRMS and LC-MS-MS analyses of dust samples revealed ∑PBDEs of 41.
View Article and Find Full Text PDFHere, we announce the draft genome sequence of sp. strain AN3A02, isolated from the rhizosphere of one of the only two species of vascular plants existing in the Antarctic continent, Desv. This isolate, which inhibited the mycelial growth of in dual culture, has a genome sequence of 6,778,644 bp, with a G+C content of 60.
View Article and Find Full Text PDFThe bacterial strain M7D1 was isolated from samples of the rhizosphere of desert bloom plants on the Atacama region located in northern Chile as part of a study intended to isolate nitrifying bacteria in this adverse environment. It was previously identified as belonging to the Pseudomonas fluorescens group. In this study, the phylogenetic analysis of the 16s RNA, gyrA, rpoB and rpoD genes confirmed that this strain belongs to this group, especially Sub Group (SG) Koreensis, but it represents a potential new species.
View Article and Find Full Text PDFFlavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout syndrome in freshwater salmonid fish worldwide, generating injuries and high mortality rates. Despite several studies on this bacterium, the infection mechanism remains unknown due to limitations in the employed animal models. In this work, we propose using zebrafish (Danio rerio) as a model for studying bacterial pathogenicity.
View Article and Find Full Text PDFWe announce the draft genome sequence of strain B2, which belongs to a potentially new species, isolated from soil associated with rhizosphere of olivillo trees (). Its size is 4,967,099 bp, and its G+C content is 49.1%.
View Article and Find Full Text PDFA Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (), designated strain T11b, was subjected to a comprehensive taxonomic investigation. A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of (98.1 %) and and (both 98.
View Article and Find Full Text PDFOne slightly beige-white pigmented, Gram-stain-negative, rod-shaped bacterium, strain I-STPP5b, was isolated from the trachea of a Gentoo penguin chick individual () investigated in Fildes Bay, Chilean Antarctic (62° 12' S, 58° 57' W). I-STPP5b consists of a 3.4 Mb chromosome with a DNA G+C content of 44.
View Article and Find Full Text PDFVibrio ordalii is an extracellular, Gram-negative bacterium that produces vibriosis in salmonids. While pathogenesis is not fully understood, this bacterium has numerous likely genes for adhesion, colonization, invasion factors and, as recently suggested, intracellular behaviour. Therefore, this study aimed to clarify possible intracellular behaviour for V.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
September 2019
An orange-pigmented, oxidase-positive bacterial strain (I-41R45), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45 with sequences of type strains of most closely related species showed highest sequence similarities to (98.
View Article and Find Full Text PDFWe announce the draft genome sequence of sp. strain M7D1, isolated from the rhizosphere of a plant in the Atacama Desert bloom event. The genome sequence had 6,170,633 bp with a G+C content of 59.
View Article and Find Full Text PDFA pink pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain 36B243), was isolated from the spleen of a black rock cod (Notothenia coriiceps, Richardson 1844) in the Chilean Antarctica. Strain 36B243 has a 5.26 Mb chromosome with a DNA G + C content of 35.
View Article and Find Full Text PDFSpring viraemia of carp (SVC) is an infectious disease responsible for severe economic losses for various cyprinid species, particularly common carp (Cyprinus carpio carpio). The causative agent is the SVC virus (SVCV), a member of the Sprivivirus genus, Rhabdoviridae family, and a List 1 pathogen notifiable by the World Organization for Animal Health. This study describes the diagnosis of an SVCV pathogen isolated in October 2015 from wild common carp inhabiting a natural lagoon in central Mexico.
View Article and Find Full Text PDFTransbound Emerg Dis
December 2018
Weissella ceti, a Gram-positive nonmotile bacterium, is currently an emerging pathogen within rainbow trout (Oncorhynchus mykiss) farms in China, Brazil, the United States, and Japan. This study is the first to isolate, identify, and characterize W. ceti isolates from rainbow trout farmed in Mexico.
View Article and Find Full Text PDFFlavobacterium psychrophilum is the etiologic agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS), which cause significant worldwide losses in aquaculture. Juvenile rainbow trout are particularly susceptible to F. psychrophilum infection, the main external clinical signs of which are extensive necrotic myositis and ulcerative lesions.
View Article and Find Full Text PDFSkeletal muscle in mammals can express and secrete immune-related molecules during pathogen infection. Despite in fish is known that classical immune tissues participate in innate immunity, the role of skeletal muscle in this function is poorly understood. To determine the immunocompetence of fish skeletal muscle, juvenile fine flounder (Paralichthys adpersus) were challenged with Vibrio ordalii.
View Article and Find Full Text PDFA slightly beige-white pigmented, Gram-staining-negative, rod-shaped bacterium, strain M1A1T, was isolated from seawater samples obtained in Fildes Bay, Antarctica (62°12' S 58° 57' W). Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.4 % 16S rRNA gene sequence identity to the type strain of Psychromonas arctica, but less than 97 % 16S rRNA gene sequence similarities to all other species of the genus Psychromonas.
View Article and Find Full Text PDFA group of seven Chilean isolates presumptively belonging to Vibrio tapetis was isolated from diseased fine flounders (Paralichthys adspersus) and red conger eel (Genypterus chilensis) experimentally reared in Quintay (Chile). All isolates were confirmed as members of V. tapetis on the basis of matrix-assisted laser desorption ionization time-of-flight MS, 16S rRNA gene sequencing, DNA-DNA hybridization values and G+C content.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
September 2016
One beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain E3/4T, was isolated from a zebrafish, Danio rerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.5 % 16S rRNA gene sequence identity to the type strain of Undibacterium macrobrachii and 97.
View Article and Find Full Text PDFVibrio ordalii, the causative agent of atypical vibriosis, is a Gram-negative, motile, rod-shaped bacterium that severely affects the salmonid aquaculture industry. V. ordalii has been biochemically, antigenically and genetically characterized.
View Article and Find Full Text PDFVibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V.
View Article and Find Full Text PDFStreptococcus phocae subsp. phocae is a pathogen that affects different pinniped and mammalian species. This announcement reports the genome sequence of the type strain ATCC 51973 isolated in Norway from clinical specimens of harbor seal (Phoca vitulina), revealing interesting genes related to possible virulence factors.
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