Characterization of anthranilic acid produced by Virgibacillus salarius MML1918 and its bio-imaging application.

Anthranilic acid (AA) holds significant importance in the chemical industry. It serves as a crucial building block for the amino acid tryptophan by manipulating the tryptophan biosynthesis pathway, it is possible to increase the production of anthranilic acid. In this study, we utilized metabolic engineering approaches to produce anthranilic acid from the halophilic bacterium Virgibacillus salarius MML1918.

Anti-fungal effects of novel N-(tert-butyl)-2-(pyridin-2-yl)imidazo[1,2-a]pyridin-3-amine derivative and it's in-vitro, in-silico, and mode of action against Candida spp.

Imidazoles are a category of azole antifungals that encompass compounds such as ketoconazole, miconazole, esomeprazole, and clotrimazole. In contrast, the triazoles group, which includes fluconazole, voriconazole, and itraconazole, also plays a significant role. The rise of antibiotic resistance in fungal pathogens has evolved into a substantial global public health concern.

Antimicrobial potential of secondary metabolites and DNA gyrase B blocking molecules produced by a halophilic bacterium Virgibacillus salarius (MML1918).

Aim: The present study aims to determine the antimicrobial potential of Virgibacillus salairus (MML1918) against human pathogens and its in-vitro and in-silico properties.

Methods And Results: In this present study, totally 63 halophilic bacterial cultures were obtained and cultivated in nutrient broth medium containing 8% NaCl and the metabolites, were extracted using ethyl acetate and screened for their antimicrobial property by cell viability assay against 12 pathogenic bacteria and fungi, among 63 halophilic bacteria the Vir. salaries (MML1918) found to be the best producer for secondary metabolites production against clinical pathogens.

Coordination of Distal Carboxylate Anion Alters Metal Ion Specific Binding in Imidazo[1,2-a]pyridine Congeners.

Imidazo[1,2-a]pyridine derivatives have excellent potential for chelation with transition metal ions. Two new imidazo[1,2-a]pyridine-8-carboxylates were synthesized and characterized by H NMR, C NMR, HRMS, and single crystal-XRD techniques. Methyl carboxylate (probe 1) turns on fluorescence upon coordination with Zn, while sodium carboxylate (probe 2) turns off its fluorescence upon coordination with Co or Cu ions present in aqueous acetonitrile medium.

Exploring DNA quantity and quality from raw materials to botanical extracts.

Objectives: The aim of this study was to explore the variability in DNA quality and quantity along a gradient of industrial processing of botanical ingredients from raw materials to extracts.

Methods: A data matrix was assembled for 1242 botanical ingredient samples along a gradient of industrial processing commonly used in the Natural Health Product (NHP) industry. Multivariate statistics was used to explore dependant variables for quality and quantity.

Chelation of specific metal ions imparts coplanarity and fluorescence in two imidazo[1,2-a]pyridine derivatives: Potential chemosensors for detection of metal ions in aqueous and biosamples.

Synthesis and chelation induced fluorescence emission from two imidazo[1,2-a]pyridine derivatives are described. The nonfluorescent molecule 1 containing N and O donor atoms, achieves coplanarity upon interactions with trivalent cations Al, Fe and Cr, that favors fluorescence emission. Molecule 2 containing two N donor atoms attains coplanarity upon interaction with the only Zn and becomes fluorescent.

Green synthesis of silver-nanoparticles from leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens.

Silver nanoparticles play a important role in controlling mosquito population as well as multi drug resistant pathogens without causing much harm to humans. In the present study was focused on green synthesis of silver nanoparticles against dengue causing vector () and pathogens affecting humans. The synthesized silver nanoparticle was confirmed using UV- absorption spectrum range obtained at 416 nm, XRD, FTIR and HR-TEM analysis were used to determine the silver nanoparticle morphology and size with ∼6.

Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens.

Antibiotic resistance is becoming a pivotal concern for public health that has accelerated the search for new antimicrobial molecules from nature. Numbers of human pathogens have inevitably evolved to become resistant to various currently available drugs causing considerable mortality and morbidity worldwide. It is apparent that novel antibiotics are urgently warranted to combat these life-threatening pathogens.

Synthesis and SAR study of novel anticancer and antimicrobial naphthoquinone amide derivatives.

A series of novel naphthoquinone amide derivatives of the bioactive quinones, plumbagin, juglone, menadione and lawsone, with various amino acids were synthesized. The compounds were characterized by (1)H NMR, (13)C NMR, Mass, IR and elemental analysis. All the compounds were evaluated for their anticancer activity against HeLa and SAS cancer cell lines and 3D-QSAR indicated the presence of electron donating group near sulphur enhanced the activity against HeLa cells.

Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates.

Fluorescence spectroscopy was examined as a potential technique for identification and classification of bacterial pathogens. Colonies of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae on agar plates were measured directly using a laboratory spectrofluorimeter coupled with optical fiber. Steady state fluorescence spectra were collected following excitation at 280 nm (tryptophan) and 380 nm (NADH).

Synthesis of 7-oxabicyclo[2.2.1]hept-5-en-2-yl derivatives and their screening for antimicrobial and antioxidant properties.

Novel 7-oxabicyclo[2.2.1]hept-5-en-2-yl derivatives have been synthesized using boron trifluoride diethyl etherate catalyzed Diels-Alder reaction.

Structural and functional studies on urease from pigeon pea (Cajanus cajan).

Urease is an enzyme that catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide, and is found in plants, microorganisms and invertebrates. Although plant and bacterial ureases are closely related at amino acid and at the structural level, the insecticidal activity is seen only in the plant ureases. In contrast, both plant and bacterial ureases exhibit antifungal activity.

Synthesis of quinoline derivatives for fluorescent imaging certain bacteria.

Highly fluorescent quinoline derivatives were synthesized using Sc(OTf)(3) catalyzed imino Diels-Alder reaction. Both the aromatic and their analogous tetradehydroquinoline derivatives were explored for the detection of bacteria using fluorescent imaging studies. Surprisingly the aromatic quinoline derivatives show a remarkable fluorescent response that can be useful in the detection of both gram positive and gram negative bacteria even at a concentration in the range of 0.

Synthesis and antimicrobial activity of some novel dicationic sulphonophanes.

The synthesis of some novel imidazole-based dicationic sulfonophanes incorporating various spacer units is described. All the sulphonophanes exhibit good antibacterial and antifungal activity against five bacterial strains Bacillus subtilis, Staphylococcus aureus, Vibrio cholera, Escherichia coli, Proteus vulgaris and human pathogenic fungus Candida albicans.

Synthesis of novel beta-lactam fused spiroisoxazolidine chromanones and tetralones as potent antimicrobial agent for human and plant pathogens.

Synthesis of novel beta-lactam fused spiroisoxazolidine chromanones and tetralones ring systems has been achieved by intermolecular 1,3-dipolar cycloaddition reaction of bicyclic nitrone with unusual dipolarophiles, arylidene chromanones/tetralones under different reaction conditions. The synthesized compounds were evaluated for antimicrobial activities. It was observed that two of the synthesized compounds exhibited relatively good antibacterial and antifungal activities.

Characterization of a thermostable alkaline protease produced by marine Streptomyces fungicidicus MML1614.

Totally 191 different marine actinomycetes were isolated from 256 different marine samples collected from the Bay of Bengal and its associated Pulicat lake and Pichavaram mangrove, India. Among them, 157 produced caseinase, 113 produced gelatinase and 108 produced both the protease enzymes. An isolate coded as MML1614 was selected for further study as it exhibited high proteolytic activity.

Synthesis of novel carbazole based macrocyclic amides as potential antimicrobial agents.

A series of carbazole based macrocyclic diamides with thia and oxy linkages have been synthesized and the inhibitory activity of the cyclophane amides against human pathogenic bacteria and plant pathogenic fungi are documented. (S)-1,10-Bi-2-naphthol [(S)-BINOL] based chiral carbazolophane amide emerged as the most interesting compound in this series exhibiting excellent antibacterial and antifungal activities.

Regioselective synthesis and antimicrobial screening of novel ketocarbazolodispiropyrrolidine derivatives.

A series of novel dispiropyrrolidine derivatives have been synthesized through 1,3-dipolar cycloaddition reaction of azomethine ylide generated from sarcosin and di/tri ketone with the dipolarophile (E)-2-arylidine-1-keto-carbazoles. The cycloadducts ketocarbazalo spiro N-methyl pyrrolidines showed the most interesting antimicrobial activity at lower concentration.

Synthesis of some novel imidazole-based dicationic carbazolophanes as potential antibacterials.

Synthesis of fluorescent imidazole-based dicationic carbazolophanes incorporating various spacer units is described. Interestingly, the cyclophanes 2a and 5a incorporating a pyridine moiety exhibited superior antibacterial activity against most of the pathogenic bacteria in the tested concentrations as compared to the other cyclophanes as well as the test control, benzalkonium chloride (BAC), cetylpyridinium chloride (CPC) and tetracycline.

Synthesis, characterization and electrochemistry of 4'-functionalized 2,2':6',2''-terpyridine ruthenium(II) complexes and their biological activity.

The synthesis and characterization of Ru(II) terpyridine complexes derived from 4'-functionalized 2,2':6',2''-terpyridine ligands by a multi step procedure have been described. The complexes are redox-active, showing both metal-centred (oxidation) and ligand-centred (reduction) processes. The antibacterial and antifungal activity of the synthesized ruthenium(II) complexes [Ru(attpy)2](PF6)2 (attpy = 4'-(4-acryloyloxymethylphenyl)-2,2':6',2''-terpyridine); [Ru(mttpy)2](PF6)2 (mttpy = 4'-(4-methacryloyloxymethylphenyl)-2,2':6',2''- terpyridine); [Ru(mttpy)(MeOPhttpy)](PF6)2 (MeOPhttpy = 4'-(4-methoxyphenyl)-2,2':6',2''-terpyridine); and [Ru(mttpy)(ttpy)](PF6)2 (ttpy = 4'-(4-methylphenyl)-2,2':6',2''-terpyridine) were tested against four human pathogens (Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa and Escherichia coli) and five plant pathogens (Curvularia lunata, Fusarium oxysporum, Fusarium udum, Macrophomina phaseolina and Rhizoctonia solani) by the well diffusion method and MIC values of the complexes are reported.

Synthesis of novel spiropyrrolizidines as potent antimicrobial agents for human and plant pathogens.

A series of novel dispirooxindolopyrrolizidine derivatives have been synthesized through 1,3-dipolar cycloaddition reaction of azomethine ylide generated from proline and isatin with the dipolarophile (E)-2-arylidine-1-keto carbazoles. The synthesized cycloadducts were evaluated for antimicrobial activities. Compounds 7d and 7e showed relatively good antibacterial and antifungal activities.

Synthesis and antibacterial activity of some novel chiral fluorophoric biscyclic macrocycles.

Synthesis of chiral permanent fluorophoric biscyclic macrocycles incorporating anthraquinone and (S)-BINOL core is described. Interestingly, the biscyclic macrocycle 1 exhibited remarkable antibacterial activity against most of the pathogenic bacteria in the tested concentrations as compared to the other three compounds 2, 14 and 17 as well as the test control, tetracycline. Further biscyclophanes 1 and 2 exhibited permanent fluorescence sensing property even under highly acidic conditions.

Morinda pubescens JE Smith (Morinda tinctoria Roxb) fruit extract accelerates wound healing in rats.

This study investigated the wound healing properties of the chloroform extract of Morinda pubescens fruit in rats. Topical application of M. pubescens fruit extract at 20 mg/mL and 10 mg/mL concentrations appreciably accelerated wound healing in rats compared with controls.

Synthesis and anti-microbial activity of pyrazolylbisindoles--promising anti-fungal compounds.

A series of pyrazolylbisindole derivatives have been synthesized by reacting substituted pyrazole aldehydes with substituted indoles using phosphotungstic acid, a Keggin type heteropoly acid as catalyst. The synthesized pyrazolylbisindoles were evaluated for anti-microbial activities. The effect of pyrazolylbisindoles on the mycelial growth of plant pathogenic fungi is revealed.

Design, synthesis and anti-microbial activity of 1H-pyrazole carboxylates.

In a SAR study, we have synthesized a few 1H-pyrazole carboxylate related microbicides using Vilsmeier reagent. The anti-microbial screening results of 1H-pyrazole-3-carboxylate are reported here for the first time. The effect of 1H-pyrazole carboxylates on the mycelial growth of plant pathogenic fungi is revealed.