Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment.

Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT-SYBR Green) was monitored in monocultures and in natural marine samples after UV irradiation (from 25 to 200 mJ/cm) and heat treatment (from 15 to 45 min at 55°C). UV irradiation of caused DNA degradation followed by the decrease in protein synthesis within a period of 24 h.

Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms.

In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active marine bacteria was performed by combining the DNA stain SYBR Green with detection of protein production with BONCAT. After optimization of incubation condition and substrate concentration on monoculture of , we applied and modified the method to natural marine samples.

Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study.

In this study, we used flow cytometry to examine how incubation in dark versus light affects the vitality and viability of UV-irradiated Tetraselmis suecica. High UV doses (300 and 400 mJ/cm) affected the esterase activity, membrane permeability, and chlorophyll content more when the subsequent incubation took place in light. For non- or low UV dose (100 and 200 mJ/cm)-treated cells, incubation in light resulted in cell regrowth as compared to incubation in dark.