Publications by authors named "Mathias Samuelsson"
Article Synopsis
- CRISPR-Cas9 technology has been successfully used for genome editing in potatoes without integrating foreign DNA, maintaining regulatory compliance.
- The study utilized a DNA-free method by delivering CRISPR-Cas9 ribonucleoproteins (RNPs) to potato protoplasts, achieving mutation rates of up to 25%.
- While the RNP approach produced transgene-free mutated lines, over 80% of these had unintended inserts, highlighting the need for further refinement in the editing process.
Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts. Site-directed mutagenesis (SDM) has shown great progress in introducing precisely targeted mutations. Engineered CRISPR-Cas9 has received increased focus compared to other SDM techniques, since the method is easily adapted to different targets.
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