A fluorescence digital image-based method using carbon quantum dots to evaluate the compliance of a biocidal agent.

In this work, a simple, low-cost and easy-to-handle analytical procedure based on carbon quantum dots (CQDs) is proposed to check commercially available formulated microbicides that are used to mitigate the transmission of viruses, such as SARS-COV-2, or bacterial diseases. For this purpose, CQDs were synthesized pyrolysis using citric acid and ethylenediamine as precursors to produce an intense fluorescence that is used to measure the concentration of hypochlorite, an important biocidal agent present in sanitizing mats, by quenching mechanisms. The characterization of the CQDs was performed using IR spectrophotometry, UV-Vis spectrophotometry, spectrofluorometry, thermogravimetric analysis, scanning electron microscopy, dynamic light scattering, X-ray diffraction, energy-dispersive spectroscopy, and zeta potential measurements.