Clinical and pathologic evidence of corticobasal degeneration and progressive supranuclear palsy in familial tauopathy.

Background: Corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP) are neurodegenerative tauopathies. Sporadic and familial cases of PSP and CBD have been noted, but both have not been reported in a single family.

Objective: To describe the clinical, oculomotor, balance, functional imaging, histopathologic, and genetic studies in a family with CBD and PSP.

[Differentiation of strains of the swine fever virus depending on their temperature resistance at 56 degrees C and on the size of fluorescent plaques].

In vitro and in vivo experiments were carried out to differentiate the virulent swine fever virus strain Vratsa and the vaccinal strains K and VP with regard to their resistance at 56 degrees C and to the size of the fluorescent plaques. The results obtained were comparable. The virulent strain was found to retain its viability at temperature of 56 degrees C for 30 min.

[The spread of enzootic bovine leukemia via the seminal fluid in certain breeds of bulls].

Studied was the occurrence of enzootic bovine leukosis as dependent on the use of semen of leukosis-affected bulls for the artificial insemination of cows and heifers and their offsprings in the F1 generation on 16 farms. Semen was used of a total of 30 bulls of the Holstein-Friesian, American Brown, and European Black-and-white breeds. The agar gel immuno-diffusion test was employed to establish antibodies to the bovine leukosis virus in the sera of the bulls.

[Diagnosis of transmissible gastroenteritis in swine by the agar gel immunodiffusion test].

A transmissive gastroenteritis antigen was obtained from intestinal content of pigs with symptoms of the disease, the presence of which was demonstrated by the agar gel immunodiffusion test. It was shown that the antigen was rough and lacking in purity, but it proved to be sensitive and specific. In the conditions of agar gel immunodiffusion in the presence of a reconvalescent serum the antigen produced a clearly expressed precipitation line for 24 to 48 hours.

[Leukemia in sheep on a state farm].

Leukosis was diagnosed in a flock of sheep. It was studied epizootiologically, clinically, morphologically, virologically, and serologically in the course of two years. Fifteen sheep manifested clinical symptoms of the disease.

[Isolation of an adequate antigen for the diagnosis of enzootic leukemia in cattle with the agar gel immunodiffusion test].

An effective antigen was obtained, presenting a leukosis virus. The culture liquid, and occasionally the cells of the FIK cell line permanently infected with the agent of bovine leukosis served as a source of virus. The antigen, possessing a very good activity was produced by means of precipitation of the virus with 30 per cent ammonium sulfate, dialysis, and additional, concentration with polyethylene glycol-4000 until a 100-fold concentrations as against the initial volume of the virus was attained.

[Use of cell agglutination with concanavalin A to detect cattle infected with the leukemia virus].

The method of cell agglutination with concanavalin 'A' was employed to study lymphocytes from the peripheral blood of cattle spontaneously infected with the virus of leukosis. The infection in the animals was demonstrated through the agar gel immunodiffusion test based on the presence of antibodies. As controls served lymphocytes of normal cattle that were serologically and clinically negative for leukosis as well as lymphocytes, of cattle that were immunized with the virus of the mucous disease and with adeno-, rota, and parainfluenza-3 viruses.

[Determination of the leukemia virus in the lymphocytes of sick cattle].

Experiments were carried out to establish the leukosis virus in lymphocytes from the peripheral blood of spontaneously infected cattle. The infected animals were detected by means of the agar gel immunodiffusion test in demonstrating the presence of leukosis virus antibodies. Lymphocytes were obtained from the blood of positively reacting animals, which were cultured at 37 degrees C for 3 to 5 days in a 'MEM' nutrient medium with the addition of 10 per cent fetal calf serum and antibiotics.

[Comparative studies of sera from cattle with complete leukemia virus and glycoprotein antigens].

One hundred cattle serums were investigated by the AGTD-test with two antigens: an antigen produced by the whole virus and an antigen containing glycoproteins. Of all serums studied 44 showed a specific precipitation in case the glycoprotein antigen was used. In case the antigen from the whole virus was used 41 serums showed a specific precipitation line, while in 3 of the serums two precipitation lines were observed.

[Serological diagnosis of bovine leukosis using the agar gel diffusion test].

Experiments were carried out to use the agar gel diffusion test (AGDT) in the study of bovine leukosis. An antigen was obtained from FLK-24 cells with a latent leukosis virus infection, which proved specific and suitable for AGDT. A total of 134 serum samples were examined applyig AGDT, for the presence of specific antibodies, originating from cattle of herds in which leukosis had been hematologically demonstrated.

[Cultivation and study of the properties of the Perego strain of the sheep poxvirus on heterologous tissue].

Strain Perego of the sheep pox virus, after 11 passages in lamb testis cultures, was successfully adapted and cultivated in 10 successive passages in a heterologous tissue--secondary cultures of newborn calf kidney. The infected cultures produced a cytopathic effect similar to that observed in a homologous culture, the difference being that part of the degenerated cells came off from the wall of the flask. At the level of its 10th passage the virus exhibited a titer of 10(3)CPE50.

[Adaptation and cultivation of the Perego strain of ovine smallpox virus in tissue culture and an evaluation of its immunogenic properties].

The strain Perego of the sheep pox virus, used for the production of the ovinized vaccine in this country, was adapted and cultured in tissue cultures of lamb testes. A total of ten passages in succession of the virus were performed with the manifestation of a characteristic cytopathic effect in the cultures. The titer of the virus was found to be within the range of 10(3.