Insertion Sequences within Oxacillinases Genes as Molecular Determinants of Resistance to Carbapenems-A Pilot Study.

Carbapenem-resistant is one of the major problems among hospitalized patients. The presence of multiple virulence factors results in bacteria persistence in the hospital environment. It facilitates bacterial transmission between patients, causing various types of infections, mostly ventilator-associated pneumonia and wound and bloodstream infections.

Evaluation of Serum Iron Parameters among Men Performing Regular Physical Activity-A Preliminary Study.

Iron deficiency anemia is one of the most common issues in clinical practice. It can be caused by intense physical activity, among other things. The aim of the study was to assess serum iron parameters in a group of men who engage in regular physical activity.

The Prevalence of Virulence Factor Genes among Carbapenem-Non-Susceptible Clinical Strains and Their Usefulness as Potential Molecular Biomarkers of Infection.

Healthcare-associated infections caused by multidrug-resistant strains are a serious global threat. Therefore, it is important to expand the knowledge on the mechanisms of pathogenicity of these particular bacteria. The aim of this study was to assess the distribution of selected virulence factor genes (, , , , , and ) among carbapenem-non-susceptible clinical isolates and to evaluate their potential usefulness as genetic markers for rapid diagnostics of infections.

Diagnostic Value of Whole-Blood and Plasma Samples in Epstein-Barr Virus Infections.

Epstein-Barr virus (EBV) is an oncogenic virus classified by the World Health Organization as a class 1 carcinogen. Post-transplant lymphoproliferative disorders are believed to be strongly related to an EBV infection. Monitoring of EBV DNAemia is recommended to assess the risk of reactivation of latent infection and to assess the effectiveness of therapy.

Comparison of Virulence-Factor-Encoding Genes and Genotype Distribution amongst Clinical Strains.

is an opportunistic pathogen encoding several virulence factors in its genome, which is well-known for its ability to cause severe and life-threatening infections, particularly among cystic fibrosis patients. The organism is also a major cause of nosocomial infections, mainly affecting patients with immune deficiencies and burn wounds, ventilator-assisted patients, and patients affected by other malignancies. The extensively reported emergence of multidrug-resistant (MDR) strains poses additional challenges to the management of infections.

Whole Blood versus Plasma Samples-How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia?

Viral infections, or their reactivations, are one of the most important groups of transplantation complications that can occur among recipients of both hematopoietic cells and solid organ transplants. They are the most commonly caused by cytomegalovirus (CMV). Currently, the use of whole blood or plasma samples is recommended for CMV viral load monitoring.

Decoding Genetic Features and Antimicrobial Susceptibility of Strains Isolated from Bloodstream Infections.

is a Gram-negative rod and an etiological factor of opportunistic infections. The infections of this etiology appear mostly among hospitalized patients and are relatively hard to treat due to widespread antimicrobial resistance. Many virulence factors are involved in the pathogenesis of infection, the coexistence of which have a significant impact on the course of an infection with a particular localization.

Conventional and Real-Time PCR Targeting Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Clinical Strains.

Multidrug-resistant , particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of this study is to examine the molecular basis of the carbapenem resistance mechanism and estimation of conventional PCR and real-time PCR usefulness for the detection of oxacillinases when compared to phenotypic carbapenemases detection.

Reliable Diagnostics of SARS-CoV-2 Infections Using One- and Two-Gene Molecular Tests for a Viral RNA Detection-Results Questioning Previous Observations.

SARS-CoV-2 is a new virus from the family and its rapid spread is now the most important medical problem worldwide. Currently used tests vary in the number and selection of SARS-CoV-2 target genes. Meanwhile, the choice of the appropriate target gene may be important in terms of a reliable detection of a viral RNA.

An Application of Imipenem Discs or ATCC 27853 Reference Strain Increases Sensitivity of Carbapenem Inactivation Method for Non-Fermenting Gram-Negative Bacteria.

Non-fermenting Gram-negative rods are one of the most commonly isolated bacteria from human infections. These microorganisms are typically opportunistic pathogens that pose a serious threat to public health due to possibility of transmission in the human population. Resistance to beta-lactams, due to carbapenemases synthesis, is one of the most important antimicrobial resistance mechanisms amongst them.

Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Clinical Strains.

is one of the most commonly isolated bacteria from clinical specimens, with an increasing isolation frequency in nosocomial outbreaks. The hypothesis tested was whether carbapenem-resistant strains display an altered carriage of the virulence factor genes, depending on the type of carbapenem resistance. The aim of the study was to investigate, by PCR, the frequency of 10 chosen virulence factors genes (, , , , , , , , and ) and the genotype distribution in 107 non-duplicated carbapenem-resistant isolates.