Differential regulation by trophic conditions of phosphorylating and non-phosphorylating NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenases in Chlorella fusca.

The two NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenases present in the green alga Chlorella fusca, namely, the phosphorylating (chloroplastic) enzyme and the non-phosphorylating (cytosolic) enzyme, are differently affected by the trophic conditions prevailing in the cell cultures. The addition of metabolizable sugars to cell cultures growing in the light promotes a marked decrease of the phosphorylating enzyme activity down to a barely detectable cellular level. In contrast, the cellular level of the non-phosphorylating enzyme is even enhanced in the presence of such sugars.

[Scanning electron microscope study of chemically disinfected endodontic files].

Forty stainless steel endodontic files were observed at scanning electron microscopy after being subjected to ten disinfection cycles of 10 minutes each one, immersed in different chemical disinfectants. Corrosion was not observed on the surface of the files in circumstances that this study was made.

[Lucilia sericata infestation of the skin openings for the bone traction device in lengthening of the tibia: apropos of a case].

Fly larvae are responsible for animal disease well known by veterinarians. However, their finding in humans in nontropical countries is a rarity. Recently, we have found several larvae of Lucilia sericata in an achondroplastic child during the phase of lengthening of both tibiae with osteotax.

[Prevention of viral infections transmitted by semen in volunteer donors for assisted reproduction. 2 years' experience].

During a 2 years period the serological markers of viral infections were evaluated in the semen of 24 donors who were involved in assisted reproduction techniques. The presence of cytomegalovirus, hepatitis B virus and human immunodeficiency virus were investigated. The results were as expected, no data of viral infection at the time of donation being found because the study population was a highly selected one.

[Immunologic abortion and its treatment by immunotherapy].

Recurrent spontaneous abortions in most cases, can be explained by classical abnormalities; but for some cases without etiology and consequently without appropriate therapy until a few years ago, there is hope a successful treatment, thanks to recent advances. Contrary to what was suspected in the past, during pregnancy, the mother is immunologically competent against the paternal antigens of the fetus; this competence is necessary for her to respond to the trophoblast paternal antigen stimulations and develop her immune tolerance. If, because of insufficient stimulation, the woman does not succeed in producing this tolerance, it is now possible to help her by vaccination with paternal lymphocytes, before she becomes pregnant.

Natural immunity to measles, rubella and mumps among Spanish children in the pre-vaccination era.

Prior to the start of mass vaccination campaigns against measles, rubella and mumps, a prevalence study of natural immunity to these diseases was undertaken in a sample of 1700 unvaccinated Spanish children. They were representative of the 3-7 year-old population in terms of age, regional distribution and urban or rural environment. Measles infection prevalence was significantly higher than that for rubella and mumps from 3 (48.

Three-year prospective study of intestinal pathogens in Madrid, Spain.

During the period July 1980 through June 1983, in a General Hospital in Madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 Salmonella spp. 506 Campylobacter jejuni, 379 Shigella spp., 12 Yersinia enterocolitica, 1,466 rotavirus, 134 Giardia lamblia, and 4 Entamoeba histolytica.

[Campylobacter neonatal enteritis (author's transl)].

Authors communicate four cases of bloody diarrhea in neonates, with Campylobacter fetus jejuni in the stools. In one case, the same bacteria was isolated from the mother and, in another case, existed possibility of a cross-infection. The mildness of the evolution is marked, specially in those cases in which maternal feeding was continued.

[Hemagglutination test and the diagnosis of food allergy].

We have studied 50 children suspected to have food allergy. Their clinical diagnoses included the following: digestive trouble (prolonged diarrhoea or vomiting), abdominal pain, repetitive urticaria, angioneurotic edema, eczema. The aim of thie study has been to value the results obtained with the hemagglutination test according to Boyden, comparing them with skin tests carried out through intradermal techniques.