Publications by authors named "Mateja Lozancic"
Surface display co-opts yeast's innate ability to embellish its cell wall with mannoproteins, thus converting the yeast's outer surface into a growing and self-sustaining catalyst. However, the efficient toolbox for converting the enzyme of interest into its surface-displayed isoform is currently lacking, especially if the isoform needs to be anchored to the cell wall near the isoform's N-terminus, e.g.
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- Yeast cell walls serve two key purposes: maintaining the physical integrity of the cell and facilitating communication with surrounding molecules and cells, requiring a balance between structural stability and adaptability.
- A study analyzed 187 proteins across 92 yeast species to identify which proteins are conserved and which are specific to certain species, revealing that many proteins involved in cell wall biosynthesis are broadly conserved except for those linked to flocculation and other unknown functions.
- The research found notable differences in the types and amounts of proteins in yeast cell walls, with specific concentration variations related to temperature changes, indicating the dynamic nature of yeast cell wall composition.
Fungal cell walls are composed of a polysaccharide network that serves as a scaffold in which different glycoproteins are embedded. Investigation of fungal cell walls, besides simple identification and characterization of the main cell wall building blocks, covers the pathways and regulations of synthesis of each individual component of the wall and biochemical reactions by which they are cross-linked and remodeled in response to different growth phase and environmental signals. In this review, a survey of composition and organization of so far identified and characterized cell wall components of different yeast genera including , , , and are presented with the focus on their cell wall proteomes.
View Article and Find Full Text PDFX chromosome STR typing has emerged recently as a powerful tool, complementary to autosomal STR typing, in solving complex forensic and missing person cases. Investigator® Argus X-12 is a commercial product that allows co-amplification of 12 X chromosomal markers belonging to four linkage groups (LGs). In this study, we analyzed by capillary electrophoresis blood samples from 100 females and 102 males from a population of northern Croatia.
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- The Investigator® Argus X-12 Kit enables the simultaneous PCR amplification of 12 X-STR markers from four linkage groups to evaluate their forensic efficiency in central Croatia.
- A study involving 200 blood samples (100 from females and 100 from males) used this kit, employing capillary electrophoresis and statistical analysis to determine allele and haplotype frequencies.
- Results indicated high polymorphism in the X-STR markers, particularly DXS10135, and demonstrated the kit's suitability for forensic applications, contributing to the establishment of a national reference X-STR database.