Transfer in vivo of tuberculin hypersensitivity by sensitised lymphocytes.

An account is given of the experimental in vivo transfer of PPD-specific delayed hypersensitivity with a very small number of autologous lymphocytes pre-sensitised with PPD-specific transfer factor (TF). Transfer was assessed in 17 volunteers both in vivo by means of the Mantoux skin reaction, and in vitro by means of the leukocyte migration inhibition test, the lymphocyte locomotion test, and the leukocyte adherence inhibition test. Positivization of the tests suggests that TF triggers a reaction which expands the effect of hypersensitivity.

Transfer in vitro of tubercolin hypersensitivity by sensitised lymphocytes.

An account is given of the experimental in vitro transfer of PPD-specific delayed hypersensitivity to peripheral leukocytes pulsed with a very small portion of autologous lymphocytes pre-sensitised with PPD-specific transfer factor (TF). Transfer was assessed by means of the leukocyte migration inhibition test, the lymphocyte locomotion test, and the leukocyte adherence inhibition test. These tests were positivized in all the experiments.

The lymphocyte locomotion assay in colon and breast cancer long survivors.

Cell-mediated immunity versus tumour antigens (cytosols) of the same histotype and site was evaluated by means of the lymphocyte locomotion (LL) assay in 46 colorectal adenocarcinoma and 41 breast ductal infiltrating carcinoma patients 36-220 months after surgical resection. Lymphocyte locomotion positivity was observed in 27/46 (58.7%) and 27/41 (65.

The LMI test in colon, breast and lung cancer long survivors.

Cell-mediated immunity towards tumour antigens (cytosols) of the same histotype and site was evaluated by means of the LMI test in long survivors after surgical resection of adenocarcinoma of the colon, infiltrating ductal carcinoma of the breast, and squamous-cell carcinoma of the lung. A positive migration index (MI less than 85.0) was observed in 17/65 (26.

Leukocyte adherence inhibition test: cell-mediated reactivity to tumor antigens in colorectal and lung tumor long survivors.

Cell-mediated immunity versus tumor antigens (cytosols) of the same histotype and site was evaluated by means of the leukocyte adherence inhibition (LAI) test in 44 colorectal adenocarcinoma and 17 lung squamous-cell carcinoma patients 3-10 years after surgical resection. LAI-positivity was observed in 17/44 (38.6%) and 13/17 (76.

Serum and salivary CEA and GICA levels in oral cavity tumours.

The gastrointestinal cancer-associated antigen (GICA) is recognised by a monoclonal antibody in both serum and tissues of patients with neoplasm of the GI tract. This study compared the serum and saliva values of carcinoembryonic antigen (CEA) and GICA in 19 healthy subjects, 43 patients with benign oral cavity lesions and 26 with histologically confirmed squamous-cell carcinomas. Serum CEA levels were much the same in all three groups, whereas salivary values were significantly higher (p less than 0.

Gastrointestinal cancer-associated antigen (GICA) in oral carcinoma.

Gastrointestinal cancer-associated antigen (GICA) is detected by means of a monoclonal antibody in the serum and pathologic tissues of patients with gastrointestinal tumors. This article compares serum and salivary GICA and carcinoembryonic antigen (CEA) levels in 19 healthy control subjects, 17 patients with benign oral cavity lesions, and 11 patients with squamous cell carcinoma of the oral cavity. Serum CEA levels were similar in all three groups, whereas salivary CEA levels were higher in patients with squamous cell carcinoma than in the control subjects (p less than 0.

[Specific immunologic stimulation induced by cryosurgery of spinocellular tumors & of the oral cavity].

In previous works the Authors have shown that cryosurgical treatment of oral squamous cell cancer modifies numerous aspecific immunological parameters mostly concerning the cell-mediated component. In the present work the Authors have studied the leukocyte migration inhibition (LMI) response in vitro of peripheral blood leukocytes from patients affected by oral squamous cancer to oral tumor antigens using the leukocyte migration agarose test. The LMI was evaluated immediately before cryosurgical treatment of oral squamous cell carcinoma and 7 and 14 days after.

[Changes in salivary immunoglobulin levels after cryosurgery of benign and malignant lesions of the oral cavity].

The authors have evaluated variations in salivary IgA, IgG, IgM levels after cryosurgical treatment of benign and malignant oral lesions. Salivary immunoglobulin levels were measured by radial immunodiffusion before a single cryotreatment 7 and 14 days after. Seven days after cryosurgery of the lesions, there was a statistically significant increase in salivary IgG and IgM levels (p less than 0,02).

[Secondary immunological response to cryosurgery in various stages of oral cavity neoplasms].

The authors have evaluated general immunocompetence after cryosurgical treatment of 33 patients affected by oral squamous carcinoma. Patients have been divided into two groups: 17 of them were classifiable in 1st and 2nd stage and 16 of them in 3rd and 4th stages. Serum immunoglobulin levels, C3 - C4, peripheral lymphocytes, E-rosette forming cells, lymphocyte response to PHA were analyzed before a single cryotreatment 7 and 14 days after.

[Humoral and cellular immune response to cryosurgery of benign and malignant lesions of the oral cavity].

The authors have evaluated general immunocompetence after cryosurgical treatment of 11 patients affected by benign oral lesions and of 17 patients affected by oral squamous carcinoma. Serum immunoglobulin levels, C3-C4, peripheral lymphocytes, E-rosette forming cells, lymphocyte response to PHA were analyzed before a single cryotreatment and 7 and 14 days after. In the patients affected by benign lesions the observed variations seem to suggest that in these cases cryosurgery has a merely mechanical action of extirpation of the lesion and the consequent extinction of the phenomena reactive to the same lesion.

[Variations of serum BuChe activity during C M F therapy].

As the Authors and Others have already demonstrated, cyclophosphamide leads to a rapid and prolonged decrease in the serum cholinesterase activity. In this work, the behaviour of the serum cholinesterase in patients subjected to C M F polychemotherapy (CTX, VCR, FU) has been studied. The grouping of patients on the basis of serum cholinesterase values before treatment has demonstrated that the enzymatic activity decrease arrives at level practically homogeneous.

[Evaluation of serum BuChe activity during polychemotherapy based on COP and C-MOPP protocols].

The Authors have evaluated the serum cholinesterase activity in patients subjected to 2 different schemes of polychemotherapy: COP (CTX, VCR, Prednisone) and C-MOPP (CTX, VCR, PCZ, Prednisone). The two schemes involve the administration of cyclophosphamide that, as has already been proved, significantly lowers serum cholinesterase level after only a few hours. The Authors have observed that the combination of cyclophosphamide with other antiblastic drugs or a longer treatment have little influence on the down curve of the serum cholinesterase in relation to what they have observed after treatment with cyclophosphamide alone.