Impact of Different Measures of Body Size on the Radiation Dose During Coronary Angiography and Percutaneous Coronary Intervention: Results from a Large Single Center Cohort.

Efforts to reduce and optimize the radiation exposure during coronary angiography and intervention have pointed at patients' body size as a major determinant of irradiation for the patients and operators. We aimed at comparing body weight and body mass index (BMI) among consecutive patients undergoing angiographic procedures (coronary angiography and/or interventions) in a single center. Patients were divided in normal weight (NW, BMI <25 Kg/m) and overweight (OW, BMI ≥25 Kg/m).

The FAST-STEMI Network in Biella From 2013 to 2019: Impact of the Delocalization of the Hospital Facilities on Ischemia Time and In-hospital Outcomes.

Background: The optimization of the strategies for myocardial revascularization has improved the outcomes of patients with ST-segment elevation myocardial infarction. In Piedmont, the FAST-STEMI regional network was created for improving the management and transportation of ST-segment elevation (STEMI) patients to primary percutaneous coronary intervention facilities, reducing the time to reperfusion. Within this network, the Hospital of Biella was delocalized in December 2014 to a new suburban structure designed for an easier access, which might have shortened the duration of patients' transportation and ischemia, with potential positive prognostic effects.

Quantification of heterogeneity to classify benign parotid tumors: a feasibility study on most frequent histotypes.

To differentiate Warthin tumors (WTs) and pleomorphic adenomas (PAs) measuring heterogeneity of intravoxel incoherent motion (IVIM) and dynamic-contrast enhanced-magnetic resonance imaging biomarkers. Volumes of interest were traced on 18 WT and 18 PA in 25 patients. For each IVIM and dynamic-contrast enhanced biomarker, histogram parameters were calculated and then compared using the Wilcoxon-signed-rank test.

Efficacy of a titanium dioxide nanoparticles - based indoor anti-odor product as assessed by electronic nose and gaschromatography-mass spectrometry.

Indoor air pollutants and odorants may have psychological and physical impact on exposed individuals and the unpleasant room air is considered as one of the factors associated with sick building syndrome comprising general symptoms such as headache and lethargy. Approaches for improving the quality of indoor air are thus important as support for human health and well-being. Photo-oxidation catalyzed by titanium dioxide (TiO), is one of the methods used for elimination of volatile organic compounds, which are the cause of odor nuisance in indoor and outdoor air.

Progress in chemical luminescence-based biosensors: A critical review.

Biosensors are a very active research field. They have the potential to lead to low-cost, rapid, sensitive, reproducible, and miniaturized bioanalytical devices, which exploit the high binding avidity and selectivity of biospecific binding molecules together with highly sensitive detection principles. Of the optical biosensors, those based on chemical luminescence detection (including chemiluminescence, bioluminescence, electrogenerated chemiluminescence, and thermochemiluminescence) are particularly attractive, due to their high-to-signal ratio and the simplicity of the required measurement equipment.

Organically modified silica nanoparticles doped with new acridine-1,2-dioxetane analogues as thermochemiluminescence reagentless labels for ultrasensitive immunoassays.

Doped organically modified silica nanoparticles (ORMOSIL NPs) with luminescent molecules represent a potent approach to signal amplification in biomolecule labeling. Herein, we report the synthesis of new ORMOSIL NPs incorporating thermochemiluminescent (TCL) 1,2-dioxetane derivatives to prepare TCL labels for ultrasensitive immunoassay, displaying a detectability comparable to those offered by other conventional luminescence-based systems. Amino-functionalized ORMOSIL NPs were synthesized for inclusion of acridine-containing 1,2-dioxetane derivatives with a fluorescence energy acceptor.

Electronic nose and chiral-capillary electrophoresis in evaluation of the quality changes in commercial green tea leaves during a long-term storage.

Electronic nose and capillary electrophoresis were applied in quality control of green tea samples subjected to long-term storage. Twelve representative green teas were considered, available as an "aged" (tea leaves stored during a long-term period of two years) and/or "not aged" (fresh products) samples. Their infusions were analyzed by an electronic nose, equipped with an array of six metal oxide semiconductor (MOS) sensors to obtain olfactive fingerprints of the volatile compounds in the infusions headspace.

Preparation and characterization of thermochemiluminescent acridine-containing 1,2-dioxetanes as promising ultrasensitive labels in bioanalysis.

Thermochemiluminescence is the luminescence process in which a thermodynamically unstable molecule decomposes with light emission when heated above a threshold temperature. We recently reported the thermochemiluminescence properties of an acridine-containing 1,2-dioxetane, which emits at relatively low temperatures (i.e.

Dioxetane-doped silica nanoparticles as ultrasensitive reagentless thermochemiluminescent labels for bioanalytics.

Thermochemiluminescence (TCL; the light emission originating by the thermally triggered decomposition of a molecule) was proposed in the late 1980s as a detection technique for immunoassays. However, after little pioneering work, this technique was abandoned because of the high temperatures required and the poor detectability in comparison to other labels. Here we describe for the first time a thermochemiluminescent acridine-based 1,2-dioxetane with a remarkably low (i.

Several approaches for vitamin D determination by surface plasmon resonance and electrochemical affinity biosensors.

This work has been focused on the development of novel optical (Surface Plasmon Resonance) and electrochemical based biosensors for the determination of 25-OH vitamin D (25OHD) which is an important factor involved in avoiding both skeletal damage and a variety of pathological conditions, and to evaluate their potential use in clinical practice. Different approaches to the determination of vitamin D using affinity based biosensors, are described herein; firstly, an immunosensor based on SPR transduction was realized for direct determination of vitamin D, obtaining a LOD of 2 μg/ml which unfortunately is too far from the needs in clinical analysis. In order to enhance the sensitivity, the vitamin D was modified with gold nanoparticles (AuNPs): the binding of 25OHD with AuNPs determines the amplification of SPR signal, allowing to lower the LOD down to 1 μg/ml, doubling the sensitivity.

Characterization and application of a diamine oxidase from Lathyrus sativus as component of an electrochemical biosensor for the determination of biogenic amines in wine and beer.

In this work, we have characterized a diamine oxidase (DAO) from Lathyrus sativus and evaluated its use, for the first time, as biocatalytic component of an electrochemical biosensor for the determination of biogenic amines index in wine and beer samples. Firstly, DAO was electrokinetically characterized free in solution by means of a platinum electrode and then immobilized by using polyazetidine prepolimer on the surface of screen-printed electrodes constituted of two gold working electrodes. The amperometric measurements were carried out by using a flow system at a fixed potential of +600 mV vs the internal silver pseudo reference in phosphate buffer solution (0.

The structure of maize polyamine oxidase K300M mutant in complex with the natural substrates provides a snapshot of the catalytic mechanism of polyamine oxidation.

Polyamine oxidases are FAD-dependent enzymes catalyzing the oxidation of polyamines at the secondary amino groups. Zea mays PAO (ZmPAO) oxidizes the carbon on the endo-side of the N5-nitrogen of spermidine (Spd) and spermine (Spm). The structure of ZmPAO revealed that the active site is formed by a catalytic tunnel in which the N5 atom of FAD lies in close proximity to the K300 side chain, the only active-site residue conserved in all PAOs.

Polyazetidine-coated microelectrodes: electrochemical and diffusion characterization of different redox substrates.

The present paper reports on the diffusion characteristics and electron transfer properties of a membrane obtained from polyazetidine prepolymer (PAP) consisting of repeating units of 1-(aminomethyl)-1-{2-[(6-oxyhexane)amino]ethyl}-3-hydroxyazetidinium chloride studied in the presence of seven simple redox electroactive molecules: ABTS, catechol, dopamine, ferrocenecarboxylic acid, ferricyanide, ferrocyanide, and the osmium complex bis(2,2-bipyridyl)-4-aminomethylpyridine chloride hexafluorophosphate (Os[(bpy)(2) 4-AMP Cl](+)). Using water as medium, the apparent diffusion coefficients (D(app)), the concentrations of the compounds in the membrane, and the heterogeneous rate constants (k(s)) were calculated as a function of temperature, and the influence thereof on these parameters was evaluated. Even if D(app) and k(s) values in the presence of PAP are smaller than in solution, this decrease is small enough to indicate that the PAP membrane shows excellent diffusion and electron-exchange properties with respect to other commonly used membranes reported in the literature.

Laccase-based biosensor for the determination of polyphenol index in wine.

In this work we have developed and characterized the use of Laccases from Trametes versicolor (TvL) and Trametes hirsuta (ThL) as biocatalytic components of electrochemical biosensors for the determination of polyphenol index in wines. Polyazetidine prepolimer (PAP) was used as immobilizing agent, multi-walled and single-walled carbon nanotubes screen-printed electrodes as sensors (MWCNTs-SPE and SWCNTs-SPE) and gallic acid as standard substrate. The amperometric measurements were carried out by using a flow system at a fixed potential of -100 mV vs.

Electron-transfer properties of short-lived N-oxyl radicals. Kinetic study of the reactions of benzotriazole-N-oxyl radicals with ferrocenes. Comparison with the phthalimide-N-oxyl radical.

A kinetic study of the one-electron oxidation of a series of substituted ferrocenes (FcX: X = H, COCH3, CO2Et, CH2OH, Et, and Me2) by the benzotriazole-N-oxyl radical (BTNO) and of ferrocene (FcH) by a series of ring-substituted benzotriazole-N-oxyl radicals (Z-BTNO: Z = H, 6-CF(3), 6-Cl, 6-Me, 6-MeO) has been carried out in CH3CN. N-Oxyl radicals were produced by hydrogen abstraction from 1-hydroxybenzotriazoles (Z-HBT) by the cumyloxyl radical produced after 355 nm laser flash photolysis of a solution of dicumyl peroxide in CH3CN. In both systems, the rate constants exhibited a satisfactory fit with the Marcus equation allowing us to determine self-exchange reorganization energy values for the BTNO/BTNO- couple, which resulted in good agreement: 34.

Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors.

A highly stable functional composite film was prepared using polyazetidine prepolymer (PAP) with peroxidase from horseradish (HRP) and/or glucose oxidase (GOx). The good permeability of the PAP layer to classical electrochemical mediators, as evaluated by the determination of the diffusion coefficient of different redox molecules, is of great importance in view of the use of PAP as an immobilizing agent in second-generation biosensor development. Cyclic voltammetry of the HRP-PAP layer on a glassy carbon electrode (GCE) showed a pair of stable and quasi-reversible peaks for the HRP-Fe((III))/Fe((II)) redox couple at about -370 mV vs.

A kinetic study of the electron-transfer reaction of the phthalimide-N-oxyl radical (PINO) with ferrocenes.

A kinetic study of the one-electron oxidation of a series of ferrocenes (FcX: X = H, CO2Et, CONH2, CH2CN, CH2OH, Et, and Me2) by PINO generated in CH3CN by reaction of N-hydroxyphthalimide (NHPI) with the cumyloxyl radical produced by 355 nm laser flash photolysis of dicumyl peroxide has been carried out. Ferrocenium cations were formed, and the reaction rate was determined by following the decay of PINO radical at 380 nm as a function of the FcX concentration. Rate constants were very sensitive to the oxidation potential of the substrates and exhibited a good fit with the Marcus equation, from which a lambda value of 38.