Publications by authors named "Massimino Jan Miele"
Article Synopsis
- The study assesses the effectiveness of thawing and washing protocols for cord blood-derived hematopoietic stem cells (HSCs), focusing on the best thawing solution and the stability of CD34+ cells over time.
- Seven cryopreserved cord blood products were tested with three different thawing solutions, revealing that 5% human albumin provided the best results for cell viability and stability.
- Results indicated that mononuclear cells remained stable for up to 4 hours after thawing, while viable CD34+ cells maintained stability for up to 6 hours, confirming the necessity of careful handling post-thaw to preserve cell functionality.
Background: Hematopoietic stem cell transplantation is commonly used to treat several oncohematologic diseases. The autologous hematopoietic progenitor cells collected through apheresis (HPC-A) must be cryopreserved and stored before use in vivo. Cell processing that precedes cryopreservation of HPC-A includes volume reduction aimed at reducing the amount of dimethyl sulfoxide used, as well as storage space.
View Article and Find Full Text PDFT regulatory cells are fundamental in the maintenance of immune homeostasis and self-tolerance. Experimental models suggest the existence of two functional types of T(reg) cells designated naturally occurring and induced. Interest in T(reg) cells increased with evidence from experimental mouse and human models demonstrating that the immunosuppressive potential of these cells can be utilized in the treatment of various pathological conditions.
View Article and Find Full Text PDFArticle Synopsis
- Umbilical cord blood (UCB) is a promising alternative for stem cell transplants, but its limited cell quantity makes it suitable primarily for low-weight patients.
- This study analyzed the outcomes of thawing 40 UCB units for patients with various blood disorders, assessing the effectiveness of the Rubinstein method of cellular manipulation before grafting.
- Results showed a high mean recovery rate of nucleated cells and good viability post-thawing, confirming the technique's efficiency and adherence to shipping protocols.