Expression of VEGFR2 Ligand Binding Domain in Pichia pink™ 4 Cells and Evaluation of Its Interactions with VEGF-A Receptor Binding Domain.

Vascular endothelial growth factor A (VEGF-A) and VEGF receptor 2 (KDR) are important mediators of angiogenesis. We aimed to express the soluble KDR ligand-binding domain (sKDR1-3) and evaluate its interaction with the VEGF-A receptor-binding domain (VEGFA-RBD). sKDR1-3 DNA was designed and subcloned into pPinkα-HC plasmid.

Clodronic Acid has Strong Inhibitory Interactions with the Urease Enzyme of : Computer-aided Design and Confirmation.

Background: (HP) infection could lead to various gastrointestinal diseases. Urease is the most important virulence factor of HP. It protects the bacterium against gastric acid.

Streptomyces strains can improve the quality properties and antifungal bioactivities of tomato fruits by impacting WRKY70 transcription factor gene and nitrate accumulation.

The current study evaluated the effect of plant growth-promoting (PGP) strains of Streptomyces on yield, quality, and nitrate content of fruits, plant-microbe responses, and antifungal effect against blight disease caused by fungus pathogen Alternaria solani on tomato fruits in commercial greenhouse conditions. Greenhouse trials were done with four treatments including strains Y28, IC10, IT25, and commercial bio-fertilizer (Barvar NPK®) on tomato plants. In PGP treatments, the number of infected fruits significantly reduced (60%) compared to Barvar and control.

Childhood trauma and type D personality: The endocrine and cardiovascular effects on stress reactivity.

Both exaggerated and blunted cardiovascular stress reactions are associated with health problems. Moreover, early life experiences and personality traits affect stress responses. Regarding the childhood traumas and type D personality, this study aimed to compare the endocrine and cardiovascular reactions against acute laboratory stress.

The comparative study of the effects of Fe O and TiO micro- and nanoparticles on oxidative states of lung and bone marrow tissues and colony stimulating factor secretion.

Nowadays, increased use of nanomaterials in industry and biomedicine poses potential risks to human health and the environment. Studying their possible toxicological effects is therefore of great significance. The present investigation was designed to examine the status of oxidative stress induced by nanoparticles (NPs) of ferric oxide (Fe O ) and titanium oxide (TiO ) with their micro-sized counterpart on mouse lung and bone marrow-derived normal tissue cells.

Data on cell survival, apoptosis, ceramide metabolism and oxidative stress in A-494 renal cell carcinoma cell line treated with hesperetin and hesperetin-7-O-acetate.

Ceramide pathway is a key regulator in cell proliferation and apoptosis and oxidative stress up-regulate ceramides. Acid ceramidase (ACDase), neutral sphingomyelinase (NSMase) and glucosylceramide synthase (GCS) are critical enzymatic systems in ceramide metabolism. Our data represent the comparative assessment of Hesperetin (Hst) and hesperetin-7-O-acetate (HTA) effects on A-494 renal carcinoma cells include cell survival, caspase-3 and 9 activities, total cellular ceramide and the activities of ACDase, NSMase, GCS and superoxide dismutase (SOD).

A new approach for separation and recovery of betaine from beet molasses based on cloud point extraction technique.

The aim of this work was to explore the possibility of the application of cloud point extraction (CPE) method in micelle media to recovery betaine from beet molasses. Response surface method was employed to assess the effects of surfactant concentration, molasses concentration, incubation time, pH, electrolyte concentration, mixing time, and surfactant type on efficiency of betaine recovery from beet molasses. Also, a mathematical model was developed to predict the effect of each variable and their interactions on the efficiency of betaine recovery.

Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase.

Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are released by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohnella A01.

New Aspects of Silibinin Stereoisomers and their 3-O-galloyl Derivatives on Cytotoxicity and Ceramide Metabolism in Hep G hepatocarcinoma Cell Line.

Ceramide as a second messenger is a key regulator in apoptosis and cytotoxicity. Ceramide-metabolizing enzymes are ideal target in cancer chemo-preventive studies. Neutral sphingomyelinase (NSMase), acid ceramidase (ACDase) and glucosyl ceramide synthase (GCS) are the main enzymes in ceramide metabolism.

Plasmin digest of κ-casein as a source of antibacterial peptides.

This study investigated the antibacterial properties of plasmin, the plasmin hydrolysis of bovine κ-casein and the fractions (named κC1, κC2, κC3, κC4, and κC5) liberated from it using RP-HPLC. The target bacteria were Escherichia coli, Staphylococcus aureus (pathogenic), Lactobacillus casei and Lactobacillus acidophilus (probiotic). Three peptides (kC1, kC3, and kC4) were found to have antibacterial activity, with κC3 peptide being the most active.

Using protein nanofibrils to remove azo dyes from aqueous solution by the coagulation process.

The ever-increasing applications of hazardous azo dyes as industrialized coloring agents have led to serious remediation challenges. In this study, proteinaceous nanofibrils were examined as coagulants for decolorization of azo dyes in aqueous solutions. The results provided some insight regarding the mechanism of dye removal.

Longitudinal follow-up of oxidative stress and DNA damage parameters in detergent workers.

Background: The aim of this study was the follow-up of work place enzyme and detergent dust exposure effects and smoking habit on DNA damage parameters of workers and the evaluation of their antioxidant enzyme activities and lipid peroxidation with regard to bag-filter installation in the work place.

Material And Methods: All investigated parameters were studied in a group of 153 workers of enzyme-free detergent production plant (E-free) and a group of 138 workers of enzyme-plus detergent plant (E-plus) and compared with 45 controls 7.2 years before and 3.

Level of oxidative stress markers among physicians in a medical residency program.

The authors investigated the effect of engaging in a medical residency program, as a stressful situation, on blood and urine levels of oxidative stress markers. Newly admitted medical residents participated in the study, along with a control group of (nonmedical) students. The authors assessed superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde, micronuclei test, sister chromatid exchange, and 8-hydroxy-2'-deoxyguanosine level.