Functional genomics of primary congenital glaucoma by pathway analysis and functional characterization of CYP1B1 mutations.

CYP1B1 is the most common gene implicated in primary congenital glaucoma (PCG) - the most common form of childhood glaucoma. How CYP1B1 mutations cause PCG is not known. Understanding the mechanism of PCG caused by CYP1B1 mutations is crucial for disease management, therapeutics development, and potential prevention.

Optimization of inclusion level of lipid in larval diet of Labeo rohita (Hamilton, 1822).

Formulation and preparation of larval feed according to the requirement of Indian major carp is a prerequisite for improving the survival (%) and growth during early developmental stages. A feeding trial of 50 days in a replicate of five was conducted to determine the optimal inclusion levels of fish oil (lipid) in the larval diet of Labeo rohita. Four isonitrogenous (50% CP) nanoparticulate diets with four lipid inclusion levels, L5 (5%), L7 (7%), L9 (9%), and L11 (11%) were prepared and fed to four groups of rohu (Labeo rohita) larvae.

Optimization of Selenium Inclusion Level in the Larval Diet of Labeo rohita.

The nutritional requirement of fish larvae remains a limiting factor in advanced aquaculture. Micronutrients are crucial for early development, but their dietary inclusion level in the larval feed of carps has not been standardized. The present study was executed to determine the optimum dietary inclusion level of organic and inorganic selenium in the larval feed of Rohu, Labeo rohita.

Effect of acclimated temperature on thermal tolerance, immune response and expression of HSP genes in Labeo rohita, Catla catla and their intergeneric hybrids.

The ability of a species and population to respond to a decrease or an increase in temperature depends on their adaptive potential. Here, the critical thermal tolerance (CTmax and CTmin) of four populations: Labeo rohita, Catla catla, and their reciprocal hybrids L. rohita♀× C.

2D DNA lattice arrays assembled from DNA dumbbell tiles using poly(A-T)-rich stems.

Poly(A-T)-rich sequences have been applied as stems of DNA dumbbell tiles for construction of single crystalline 2D DNA lattice arrays in slightly acidic solutions. These arrays show much higher stability and better organised crystalline lattice structures than those assembled from DNA dumbbell tiles with randomly sequenced stems in slightly alkaline environments. DNA nanotechnology probably provides a useful platform to study the mechanical properties of DNA duplexes with specific sequences.

DNA dumbbell tiles with uneven widths for 2D arrays.

Small DNA tiles built from linear oligonucleotides for the construction of DNA nanostructures often have even widths in the lateral direction against the DNA helical axis. Herein we present an example of DNA dumbbell tiles with uneven widths between the central double helical stem and the two head loops after association with helper strands. A characteristic of DNA dumbbell tiles is that the stem length controls the stereostructure of the two head motifs.

DNA nanotubes assembled from tensegrity triangle tiles with circular DNA scaffolds.

Using small circular DNA molecules of different lengths as scaffolds, we successfully synthesised DNA nanotubes consisting of Mao's DNA tensegrity triangle tiles with four-arm junctions (Holliday junctions) at all vertices. Due to the intrinsic curvature of the triangle tile and the consecutive tile alignment, the 2D arrays are organised in the form of nanotubes. Two sized triangle tiles with equilateral side lengths of 1.