Hemoglobin E syndromes in Pakistani population.

Background: Hemoglobin E is an important hemoglobin variant with a worldwide distribution. A number of hemoglobinopathies have been reported from Pakistan. However a comprehensive description of hemoglobin E syndromes for the country was never made.

An insight into the suspected HbA2' cases detected by high performance liquid chromatography in Pakistan.

Background: Hemoglobin A2' (delta 16 Gly → Arg) is globally the commonest delta chain variant of HbA2. It is clinically and hematologically silent but its sole importance lies in the underestimation of HbA2 quantity during the workup of β-thalassaemia trait. High performance liquid chromatography (HPLC) identifies it as a small S-window peak with a mean retention time of 4.

Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: a study of 129 samples.

Objectives: To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in patients with type I and II diabetes having hemoglobin D.

Design And Methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA(1c) estimation in patients with homozygous and heterozygous D disease.

Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA(1c) estimation.

Chromatographic analysis of Hb S for the diagnosis of various sickle cell disorders in Pakistan.

Sickle cell disease remains a relatively obscure theme in research on haemoglobinopathies in Pakistan. Limited data is available regarding its prevalence in the country. The objective of our study was not only to estimate the frequency of different sickle cell diseases but also to provide quantitative estimation of haemoglobin S and other haemoglobin variants using an automated high-performance liquid chromatography (HPLC) system.

Identification of hemoglobin Q India (alpha 1-64 Asp-His) through ARMS-PCR. First report from Pakistan.

Various hemoglobinopathies have been reported from Pakistan excepting the rare ones like hemoglobin Q India. Our purpose of study was to identify the mutation (alpha 1 64 aspartate to histidine) through amplification restriction mutation system-polymerase chain reaction (ARMS-PCR) in patients where hemoglobin Q has been detected via high performance liquid chromatography (HPLC) and also to evaluate the cost effectiveness of the two technologies. All patients irrespective of age and gender who underwent HPLC for identification of their hemoglobin variant during January 1, 2006 to January 30, 2007 were studied.