The Rising Tide of Antibiotic Resistance: A Study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae.

Background: The global spread of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Enterobacterales (CRE) poses a significant concern. Acquisition of antimicrobial resistance genes leads to resistance against several antibiotics, limiting treatment options. We aimed to study ESBL-producing and CRE transmission in clinical settings.

Revolutionizing and identifying novel drug targets in via subtractive proteomics and development of a multi-epitope vaccine using reverse vaccinology and immuno-informatics.

is a gram-negative rod that has been linked to infections in people with significant comorbidities and immunocompromised immune systems. It is most commonly known to cause urinary tract infections. Thus, the development of an efficacious vaccine is imperative, as the pathogen has acquired resistance to current antibiotics.

Antibacterial efficacy of indigenous Pakistani honey against extensively drug-resistant clinical isolates of Salmonella enterica serovar Typhi: an alternative option to combat antimicrobial resistance.

Background: Extensively drug-resistant (XDR) Salmonella enterica serovar Typhi (S. Typhi) poses a grave threat to public health due to increased mortality and morbidity caused by typhoid fever. Honey is a promising antibacterial agent, and we aimed to determine the antibacterial activity of honey against XDR S.

study to predict promiscuous T cell and B cell-epitopes derived from the vaccine candidate antigens of binding to MHC class-II alleles.

Malaria is one of the major causes of health and disability globally, even after tremendous efforts to eradicate it. Till date no highly effective vaccine is available for its control. The primary reason for the low efficacy of vaccines is extensive polymorphism in potential vaccine candidate antigen genes and HLA polymorphisms in the human population.

Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant .

, a Gram -ve, rod-shaped, and opportunistic bacteria isolated from the urine, feces, and skin of humans engage in a wide range of infectious diseases such as urinary tract infection (UTI), gastroenteritis, and bacteremia. This bacterium belongs to the Enterobacteriaceae family and can resist antibiotics known as multidrug-resistant (MDR), and as such can be life-threatening to humans. After retrieving the whole proteomic sequence of ATCC 35613, a total of 6 non-homologous and pathogenic proteins were separated.

Green Synthesis of Silver Nanoparticles Using the Plant Extract of and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches.

In this study, the antibacterial and antifungal properties of silver nanoparticles synthesized with the aqueous plant extract of leaves were defined using a simplistic, environmentally friendly, reliable, and cost-effective method. The aqueous plant extract of , which served as a capping and reducing agent, was used to biosynthesize silver nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to analyze the biosynthesized silver nanoparticles (AgNPs).

Phytochemical Investigation, Antioxidant Properties and In Vivo Evaluation of the Toxic Effects of .

Parthenium hysterophorus L. is a poisonous Asteraceae weed. The phytochemical profile, antioxidant activity, total phenolic contents (TPC), total flavonoid contents (TFC), and cytotoxicity of Parthenium hysterophorus L.

Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated from (buffalo) Milk as a Probiotic.

The demand of functional foods is on the rise, and researchers are trying to develop nutritious dairy products by using well-characterized strains of bacteria. In this study, we identified locally isolated strains of Lactobacillus fermentum from Bubalus bubalis (Nilli Ravi buffalo) milk and evaluated their potential as probiotics in food products like fermented milk. Fifteen Lactobacillus strains were initially isolated, and only four strains (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were examined for morphological and biochemical characterizations due to their ability of gas production in Durham tubes.

Antifungal potential, chemical composition of and SEM analysis of morphological changes in .

In pursuit of an environmentally benign fungicide alternative, the current study explored the antifungal activity of extracts against six plant pathogenic fungi (). The well diffusion agar method was used to investigate the growth inhibition of , sp., , , , and using the three extracts viz.

Antioxidant, antibacterial, and antimutagenic activity of seeds extracts.

is a widely used plant in traditional remedies and known for its numerous biological properties. However, fraction-based antioxidant activity and their antimutagenic potential are not yet fully investigated. Different extracts of the seeds were obtained by sequential extraction in different solvents.