Publications by authors named "Masatoshi Hozaki"
Polarized light retardation analysis allows for the evaluation of tension in individual stress fibers.
Biochem Biophys Res Commun
September 2022
Article Synopsis
- Tension in stress fibers (SFs) is crucial for various biological processes like cell movement and protein synthesis.
- Previous research methods using live cells may have inaccurately measured contraction forces due to increased polarized light retardation.
- This study confirmed that SF contraction leads to increased light retardation regardless of cell activity and found that the density of SFs increases as they contract, leading to a reduction in diameter while maintaining nearly constant length.
Vascular smooth muscle cells (VSMCs) have two distinct phenotypes: contractile and synthetic. The major difference between these phenotypes lies in the magnitude of the contractile force produced by the cell. Although traction force microscopy (TFM) is often used to evaluate cellular contractile force, this method requires complex preprocessing and a sufficiently compliant substrate.
View Article and Find Full Text PDF