[Surveillance on Pseudomonas aeruginosa isolated in Gifu prefecture (2004)].

We analyzed Pseudomonas aeruginosa isolates in Gifu prefecture between September and October 2004. We conducted antimicrobial susceptibility test for 266 strains isolated from 8 medical institutes and 1 clinical laboratory, based on broth microdilution method. The MIC50 and MIC90 of piperacillin, amikacin, imipenem, and ciprofloxacin were 4 and 64, 4 and 8, 1 and 16, 0.

[Surveillance based on molecular epidemiology for Haemophilus influenzae Isolates in Gifu Prefecture].

We analyzed Haemophilus influenzae isolates in Gifu prefecture between May 2003 and August 2003. We conducted molecular-level epidemiological studies for 313 strains using PCR to identify resistant genes in H. influenzae.

[Epidemiology of Streptococcus pneumoniae isolates in Gifu Prefecture].

We analyzed Streptococcus pneumoniae isolates confirmed by direct PCR in Gifu prefecture between May 2002 and August 2002. We analyzed isolates of 254 strains from 6 hospitals to determine antibiotic susceptibility, genotype of penicillin-binding protein (PBP) genes and macrolide resistant genes, and the serotypes distribution of isolates from Matsubara Otorhinolaryngology Clinic. Isolates in which abnormal PBP genes of pbp1a, pbp2x, and pbp2b were identified by PCR were classified based on PCR results as follows; (i) penicillin-susceptible (PSSP) with 3 normal PBP genes, (ii) penicillin-intermediate (PISP) with an abnormal pbp2x, (iii) PISP with an abnormal php2b, (iv) PISP with abnormal pbp2x and pbp2b, (v) PISP with abnormal pbpla and pbp2x, (vi) penicillin-resistant (PRSP) with 3 abnormal PBP genes.