Publications by authors named "Masaru Hakoda"
Our previous studies revealed that the dielectrophoresis method is effective for separating cells having different dielectric properties. The purpose of this study was to evaluate the separation characteristics of two kinds of cells by direct current (DC) voltage offset/alternating current (AC) voltage using an insulating porous membrane dielectrophoretic separator. The separation device gives dielectrophoretic (DEP) force and electrophoretic (EP) force to dispersed particles by applying the DC-offset AC voltage.
View Article and Find Full Text PDFCorrelation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.
Bioprocess Biosyst Eng
September 2013
Article Synopsis
- The study aims to create a system for analyzing cell activity through dielectrophoresis, focusing on mouse hybridoma 3-2H3 cells.
- Previous research indicated a link between cell growth activity and dielectric properties, suggesting that these properties could evaluate stem cell activity without inducing differentiation.
- The findings showed that while both AC and DC electric fields impact cell viability, the AC field has comparatively less negative effect on growth activity and viability of the cells.
Separation of viable and nonviable animal cell using dielectrophoretic filter.
Biotechnol Prog
December 2010
Article Synopsis
- Selective separation of cells using dielectrophoresis (DEP) has limitations for large-scale applications due to inefficiencies near electrodes.
- A DEP filter was evaluated for its effectiveness in separating viable and nonviable animal cells, focusing on the impacts of AC electric field frequency and squared electric field intensity.
- Results showed a distinct frequency response for the different cell types, leading to an empirical equation for optimizing DEP separation conditions like voltage and flow rate.
Separation characteristics of animal cells using a dielectrophoretic filter.
Bioprocess Biosyst Eng
June 2010
The separation characteristics of a wire-wire type dielectrophoretic (DEP) filter were evaluated using animal cells. The separation of cells with different activities was examined using a DEP filter. The specific growth rate of the cells in retention liquid was larger than that in permeation liquid.
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