Purpose: Prostaglandins have been implicated as endogenous modulators of bladder function under physiological and pathological conditions. We examined how the expression of each EP receptor subtype changed in association with bladder outlet obstruction and focused on the functional role of EP4 receptor subtype in the bladder with outlet obstruction.
Materials And Methods: We assessed the gene expression of EP receptor subtypes by reverse transcriptase-polymerase chain reaction.
Objectives: To examine the possibility that mechanosensitive ion channels, including epithelial Na+ channels (ENaC), are implicated in mechanosensory transduction of the rat urinary bladder.
Methods: Cystometry with continuous infusion was performed to investigate the effect of intravesically perfused amiloride (a blocker of ENaC) on micturition reflex in urethane-anesthetized female rats. Bladder strips with or without epithelium suspended in organ bath were subjected to varying degrees of mechanical stretch (up to 50%).