γ-Cyclodextrin-polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution.

Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.

Purification and concentration of non-infectious West Nile virus-like particles and infectious virions using a pseudo-affinity Cellufine Sulfate column.

Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) antigens via Cellufine Sulfate column chromatography was examined. Virus-like particles (VLPs) produced by the expression of the prM and E genes were separated from most of the contaminant proteins with 0.

Selective removal of endotoxin from a DNA solution by cross-linked cyclodextrin beads.

The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 µg mL(-1), pH 6, ionic strength μ = 0.

Limulus amebocyte lysate assay for endotoxins by an adsorption method with polycation-immobilized cellulose beads.

To assay lipopolysaccharides (LPSs) in solutions containing Limulus amebocyte lysate (LAL)-inhibiting or LAL-enhancing compounds, we developed a selective endotoxin (LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and LAL. The PL-Cellufine can adsorb LPSs in a solution containing certain compounds (NaCl, proteins and amino acids) at an ionic strength of mu = 0.05-0.

Selective assay for endotoxin using poly(epsilon-lysine)-immobilized Cellufine and Limulus amoebocyte lysate (LAL).

We developed a selective endotoxin (lipopolysaccharide; LPS) assay using poly(epsilon-lysine)-immobilized cellulose beads (PL-Cellufine) and Limulus amoebocyte lysate (LAL). First, LPS was selectively adsorbed on the beads in a solution containing various LAL-inhibiting or LAL-enhancing compounds (e.g.