A proposed combination of flat-panel detector and mobile X-ray systems for low-dose image-guided central venous catheter insertion.

A central venous catheter (CVC) should be inserted at the optimum position to infuse medicines, blood products, nutrients, or fluids. Positioning of the catheter tip is commonly performed under landmark or fluoroscopic guidance. However, Japanese regulations do not allow the performing of fluoroscopy-guided procedures outside of the fluoroscopy room.

Measurement of the half-value layer for CT systems in a single-rotation technique: Reduction of stray radiation with lead apertures.

Purpose: This study aimed to compare two methods of assessing the half-value layer (HVL) for computed tomography scanners in a single-rotation technique with and without lead apertures (SRTLA / SRT).

Methods: A 0.6 cc real-time ionization chamber was suspended freely in the air at the isocenter, and six sheets of lead (130 × 170 × 2 mm) were placed at the bottom of the gantry cover, forming five apertures each having a width of 16 mm (SRTLA geometry).

Expression profile of ribosomal protein L10a throughout gonadal development in rainbow trout (Oncorhynchus mykiss).

Ribosomal protein L10a (RpL10A) has been previously established as a stimulator during the early stages of ovarian development in both the banana prawn and the fruit fly. In order to develop a greater understanding of the role of this protein in vertebrates, the present study aimed to characterize the expression profile of rpl10a during gonadal development in fish. It was determined that the expression of rpl10a within genital ridges increased during embryonic development.

Combining next-generation sequencing with microarray for transcriptome analysis in rainbow trout gonads.

Microarray technology is a powerful tool for studying genome-wide gene expression. As the genome of many fish has not yet been determined, however, cDNA microarrays can only be designed from limited expressed sequence tag data. In this study, we designed a microarray based on the sequencing data (337,466 reads) obtained by next-generation sequencing of RNA extracted from rainbow trout (Oncorhynchus mykiss) embryonic genital ridge, testis, and ovary.

Flow-cytometric isolation and enrichment of teleost type A spermatogonia based on light-scattering properties.

The transplantation of germ cells is a powerful tool both for studying their development and for reproductive biotechnology. An intraperitoneal germ cell transplantation system was recently developed for use in several teleost species. Donor germ cells transplanted into the peritoneal cavity of hatchlings migrated toward and were incorporated into the recipient's genital ridges, where they underwent gametogenesis.