Purification, characterization, and cloning of the gene for a biodegradable plastic-degrading enzyme from Paraphoma-related fungal strain B47-9.

Paraphoma-related fungal strain B47-9 secreted a biodegradable plastic (BP)-degrading enzyme which amounted to 68 % (w/w) of the total secreted proteins in a culture medium containing emulsified poly(butylene succinate-co-adipate) (PBSA) as sole carbon source. The gene for this enzyme was found to be composed of an open reading frame consisting of 681 nucleotides encoding 227 amino acids and two introns. Southern blot analysis showed that this gene exists as a single copy.

Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants.

To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-butylene adipate) (PBSA) and poly-(butylene succinate) (PBS) films. The strain grew on the surface of soil-mounted BP films, produced breaks along the direction of hyphal growth indicated that it secreted a BP-degrading enzyme, and has directly contributing to accelerating the degradation of film.

One of two major paralogs of AVR-Pita1 is functional in Japanese rice blast isolates.

We analyzed the avirulence gene AVR-Pita1 in Japanese rice blast isolates to determine how they gain virulence toward rice cultivars containing the Pita resistance gene. An avirulent isolate, OS99-G-7a (G7a), from a Japanese commercial field contained two paralogs of AVR-Pita1, designated as AVR-Pita1(JA) and AVR-Pita1(JB). Analysis of virulent, independent mutants derived from G7a, a single avirulent progenitor strain, indicated that AVR-Pita1(JA) was functional but AVR-Pita1(JB) was nonfunctional.

Co-inoculation of an antibiotic-producing bacterium and a lytic enzyme-producing bacterium for the biocontrol of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici.

The antifungal compound 2,4-diacetylphloroglucinol-producing bacterium, Pseudomonas fluorescens strain LRB3W1, inhibits the growth of Fusarium oxysporum f. sp. lycopersici, and controls Fusarium wilt of tomato caused by F.

Comparative analysis of argK-tox clusters and their flanking regions in phaseolotoxin-producing Pseudomonas syringae pathovars.

DNA fragments containing argK-tox clusters and their flanking regions were cloned from the chromosomes of Pseudomonas syringae pathovar (pv.) actinidiae strain KW-11 (ACT) and P. syringae pv.

Combined use of the biocontrol bacterium Pseudomonas fluorescens strain LRB3W1 with reduced fungicide application for the control of tomato Fusarium wilt.

Pseudomonas fluorescens strain LRB3W1 inhibited the mycelial growth of Fusarium oxysporum f. sp. lycopersici and suppressed the Fusarium wilt of tomato.