Publications by authors named "Masako Ikeuchi"
Article Synopsis
- The study examined how rat bone marrow stromal cells (MSCs) differentiate into bone-forming cells on hydroxyapatite disks with varying porosities (30%, 50%, and 70%).
- MSCs treated with dexamethasone showed increased alkaline phosphatase activity and osteocalcin production, indicating enhanced bone formation, particularly on the more porous disks.
- In vivo, bone grafts with higher porosity (HA50 and HA70) led to greater bone formation over time compared to HA30, highlighting that scaffold porosity significantly affects the osteogenic potential of implanted MSCs.
Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1 and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time.
View Article and Find Full Text PDFWe developed fully opened interconnected porous calcium hydroxyapatite ceramics having two different pore sizes. One has pores with an average size of 150 microm in diameter, an average 40-microm interconnecting pore diameter, and 75% porosity (HA150). The other has pores with an average size of 300 microm in diameter, an average 60-100-microm interconnecting pore diameter, and 75% porosity (HA300).
View Article and Find Full Text PDFArticle Synopsis
- * Five types of HA composites were tested: HA alone, GDF/HA, MSCs/HA, GDF/MSCs/HA, and BMP/MSCs/HA, with only the GDF/MSCs/HA showing significant bone formation by 2 weeks.
- * GDF-MS/HA composites enhanced bone growth compared to other combinations, indicating they could be promising for use as bone grafts in tissue engineering.
Rat and human bone marrow cells (BMCs) were cultured on a composite ceramic of zinc-containing beta-tricalcium phosphate and hydroxyapatite (ZnTCP/HAP) with a (Ca+Zn)/P molar ratio of 1.60 and varying zinc contents. After a 2-week culture of the BMCs in the presence of beta-glycerophosphate and dexamethasone, many macroscopic mineralized areas with high alkaline phosphatase (ALP) activity were seen on the ZnTCP/HAP ceramic disks.
View Article and Find Full Text PDFWe evaluated the combination effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and cultured rat bone marrow mesenchymal stem cells (MSCs) in atelopeptide type I collagen (AC) solution on osteogenesis in a diffusion chamber (DC) to develop a bone substitute having consistent osteogenic capability for clinical applications. The cultured MSCs were obtained by 10-day primary culture of fresh bone marrow cells of Fischer rats. We prepared three groups of DCs: AC solution with rhBMP-2, AC solution with cultured MSCs, and AC solution with rhBMP-2 and cultured MSCs.
View Article and Find Full Text PDF