Glucose oxidase, horseradish peroxidase and phenothiazine dyes-co-adsorbed carbon felt-based amperometric flow-biosensor for glucose.

To develop an amperometric flow-biosensor for glucose, the stabilizing effect of methylene blue (MB) toward adsorbed glucose oxidase (GOx) on carbon felt (CF) was successfully applied to prepare the GOx-modified CF-based enzyme reactor combined with a horseradish peroxidase (HRP)-modified CF-based HO detector. Upon mixing MB in the GOx-adsorption solution, the O-dependent GOx-activity was significantly increased with increasing concentration of MB in the GOx-adsorption solution. The GOx-immobilization protocol on CF is very straightforward [, adsorption of the GOx/MB mixed aqueous solution for 5 min under ultrasound (US)-irradiation].

Carbon felt-based bioelectrocatalytic flow detectors: highly sensitive amperometric determination of hydrogen peroxide using adsorbed peroxidase and thionine.

Horseradish peroxidase (HRP) and thionine (TN) were co-adsorbed onto a porous carbon felt (CF), and the resulting HRP and TN-adsorbed CF (HRP-TN-CF) was successfully used as a working electrode unit of a novel bioelectrocatalytic flow detector for a highly sensitive amperometric determination of hydrogen peroxide (H(2)O(2)). Co-adsorbed TN was essential to enhance the cathodic peak current of H(2)O(2), and the current responses of the HRP-TN-CF-based detector were much larger than those of the HRP-CF-based detector (without TN). When air-saturated 0.