Single-chamber microbial fuel cells (MFCs) were constructed using rice bran (carbon source) and pond bottom mud (microbial source). The total electric charge obtained in the MFC combining rice bran with pond bottom mud was four times higher than that in MFC using only rice bran. Phylogenetic analyses revealed dominant growth of fermentative bacteria such as and species, and exoelectrogenic species in the anode biofilms, suggesting that mutualism of these bacteria is a key factor for effective electricity generation in the MFC.
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