Publications by authors named "Masahki Matsudaira"
The light-driven splitting of water to oxygen (O) is catalyzed by a protein-bound tetra-manganese penta-oxygen calcium (MnOCa) cluster in Photosystem II. In the current study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to perform two-dimensional imaging of light-induced O evolution from spinach leaves. The employed Bio-LSI chip consists of 400 sensor electrodes with a pitch of 250 μm for fast electrochemical imaging.
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- Multiplexed bioimaging systems enhance biological assays by providing new insights, but monitoring multiple cell functions poses challenges.
- A novel electrochemical imaging system using a large-scale integration amperometric device has been developed to detect multiple biomolecules at once, creating multicolor electrochemical images.
- This system allows for real-time mapping of various cell functions, marking a pioneering approach to analyzing multiple electroactive species simultaneously.
Motion tracking of microorganisms is useful to investigate the effects of chemical or physical stimulation on their biological functions. Herein, we describe a novel electrochemical imaging method for motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device. The device consists of 400 electrochemical sensors with a pitch of 250 μm.
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- The paper discusses a new method of bioimaging that uses a large-scale integration (LSI) electrochemical device with 400 sensors to measure enzyme activity through potentiometric detection.
- This technique allows for the detection of enzymes like glucose oxidase (GOx) and alkaline phosphatase (ALP) without affecting cell activities, maintaining the integrity of the biological samples.
- The research successfully demonstrates real-time imaging of enzyme activity in hydrogels and embryonic stem cells, marking a significant advancement in using LSI-based devices for bioimaging.
In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.
View Article and Find Full Text PDFIn the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 μm for rapid electrochemical imaging of large areas. PC12 spheroids were stimulated with K(+) to release dopamine.
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- Researchers developed the "Bio-LSI," a large-scale integrated CMOS-based sensor array with 400 measurement points for electrochemical bio-imaging and multi-point biosensing.
- The new chip features a light-shield structure that drastically reduces noise from photocurrent to under 1%, and includes a mode-selectable function allowing individual control of 400 electrodes for various measurement modes.
- Demonstrations showed the system's effectiveness by creating images from redox reactions, detecting O2 and H2O2 simultaneously, and modifying sensors at selected electrodes, highlighting its potential for diverse analytical applications.
We have developed an LSI-based amperometric sensor called "Bio-LSI" with 400 measurement points as a platform for electrochemical bio-imaging and multi-point biosensing. The system is comprised of a 10.4 mm × 10.
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