Molecular characterization and gene expression of Japanese eel (Anguilla japonica) gonadotropin receptors.

A luteinizing hormone receptor (lhr) cDNA with high identity to other fish lhrs was fully cloned from the ovary of the Japanese eel (Anguilla japonica). The genes for two gonadotropin receptors (Gthr), follicle-stimulating hormone receptor (fshr) and lhr, were differentially expressed during oogenesis, which was artificially induced by salmon pituitary extract, a gonadotropin-rich source. Transcript abundance of fshr was significantly elevated at the early vitellogenic stage and peaked at the late vitellogenic stage, while lhr gene expression rapidly induced at the late vitellogenic stage and thereafter remained at a high level.

Oligonucleotide bearing ethylenediamine-N,N,N'-Triacetates for gap-selective DNA hydrolysis by Ce4+/EDTA.

With the use of two oligonucleotides bearing ethylenediamine-N,N,N'-triacetate groups as additives, gap sites were formed at predetermined sites in substrate DNA. Upon treating these systems with a Ce(4+)/EDTA complex at pH 7.0 and 37 degrees C, the phosphodiester linkages at the gap site were selectively hydrolyzed.

Occurrence of Vibrio vulnificus in fish and shellfish available from markets in China.

Vibrio vulnificus is a naturally occurring estuarine bacterium often associated with disease such as septicemia in humans following consumption of raw and lightly cooked seafood. In China and neighboring countries, rapid economic growth has encouraged increased consumption of seafood, and dietary habits are changing, with more people eating raw fish. In this study, the prevalence of V.

Site-selective DNA hydrolysis by the combination of Ce(IV) and oligonucleotide bearing EDTA groups.

By using appropriately modified oligonucleotides, a gap-structure is formed in substrate DNA, and 2-6 ethylenediamine-N,N,N'-triacetate residues are placed near this gap. When these composites are treated with homogenous Ce(IV)/EDTA complex, the phosphodiester linkages in the gap-site are selectively hydrolyzed at much greater rates than achieved previously by using unmodified oligonucelotides.