Publications by authors named "Masahiro Honjo"

A more detailed understanding of the mechanisms underlying the formation of microbial communities is essential for the efficient management of microbial ecosystems. The stable states of microbial communities are commonly perceived as static and, thus, have not been extensively examined. The present study investigated stabilizing mechanisms, minority functions, and the reliability of quantitative ana-lyses, emphasizing a metabolic network perspective.

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Article Synopsis
  • The study examined the decline of Comamonas testosteroni R2's ability to degrade phenol in chemostat conditions and its aerobic growth in batch settings, identifying physiological and transcriptomic changes.
  • It was found that the accumulation of metabolites led to decreased enzymatic activities for phenol and catechol degradation and a significant up-regulation of genes for nitrogen transport, particularly ammonium uptake, under collapse conditions.
  • The research demonstrated that amino acids like L-arginine can reverse growth inhibition by balancing carbon and nitrogen metabolism, indicating that this imbalance contributed to the strain's collapse.
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The coexisting mechanism of a synthetic bacterial community (SBC) was investigated to better understand how to manage microbial communities. The SBC was constructed with three kinds of phenol-utilizing bacteria, Pseudomonas sp. LAB-08, Comamonas testosteroni R2, and Cupriavidus sp.

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A batch culture was enriched on phenol with trichloroethene-contaminated aquifer soil as an inoculum. Cupriavidus sp. strain P-10 was isolated from the culture using a diluted plating method.

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