Attenuation of chronic thermotolerance by KNK437, a benzylidene lactam compound, enhances thermal radiosensitization in mild temperature hyperthermia combined with low dose-rate irradiation.

Purpose: We investigated whether the attenuation of chronic thermotolerance by KNK437, a heat shock protein inhibitor, can modify the effect of thermal radiosensitization in mild temperature hyperthermia (MTH) combined with low dose-rate irradiation (LDRI).

Materials And Methods: The human lung adenocarcinoma cell line A549 was simultaneously exposed to LDRI with MTH at 41 degrees C and KNK437 at a dose of 100 microM. Cell survival was estimated by a clonogenic assay.

Monitoring mitochondrial metabolisms in irradiated human cancer cells with (99m)Tc-MIBI.

Cationic hexakis(2-methoxyisobutylisonitrile)-technetium-99m ((99m)Tc-MIBI), an agent for scintigraphic detection and imaging of tumors, accumulates in mitochondria of various cells and tissues of high mitochondrial metabolic activity. To monitor the mitochondrial metabolisms of human cancer cells exposed to ionizing radiation, uptake of (99m)Tc-MIBI in an irradiated human lung cancer cell line (A549) was measured at 1-12 h following 0-9 Gy irradiation in vitro. Mitochondrial membrane potential, an index of mitochondrial activity, was also determined by flow cytometry with 3,3'-dihexyloxacarbocyanine (DiOC6(3)).

A case of venous thoracic outlet syndrome recognized by arm swelling.

Most cases of thoracic outlet syndrome are detected by neulogical symptoms, and most of the other symptoms are caused by arterial stenosis. It is rare for the syndrome to be recognized by venous symptoms. We report a 56-year-old woman with thoracic outlet syndrome recognized by arm swelling.