Click3D: Click reaction across deep tissues for whole-organ 3D fluorescence imaging.

Click chemistry offers various applications through efficient bioorthogonal reactions. In bioimaging, pretargeting strategies have often been used, using click reactions between molecular probes with a click handle and reporter molecules that make them observable. Recent efforts have integrated tissue-clearing techniques with fluorescent labeling through click chemistry, allowing high-resolution three-dimensional fluorescence imaging.

Transient sensorimotor projections in the developmental song learning period.

Memory recall and guidance are essential for motor skill acquisition. Like humans learning to speak, male zebra finches learn to sing by first memorizing and then matching their vocalization to the tutor's song (TS) during specific developmental periods. Yet, the neuroanatomical substrate supporting auditory-memory-guided sensorimotor learning has remained elusive.

Heterogenous expression of endoglin marks advanced renal cancer with distinct tumor microenvironment fitness.

Intratumoral heterogeneity, including in clear cell renal cell carcinoma, is a potential cause of drug resistance and metastatic cancer progression. We specified the heterogeneous population marked by endoglin (also known as CD105) in a preclinical model of clear cell renal cell carcinoma progression. Highly malignant derivatives of human clear cell renal cell carcinoma OS-RC-2 cells were established as OS5Ks by serial orthotopic inoculation in our previous study.

Simple method to measure and analyze the fluctuations of a small particle in biopolymer solutions.

We developed a simple method to investigate the motion of a small particle in biopolymer solutions. Using optical tweezers with low stiffness, a trapped probe particle fluctuates widely for a long time along the light axis, which reflects the rheological properties of the surrounding environment. We present a convenient technique for three-dimensional position tracking and the analysis focused on the distribution of particle positions and its variance in a given time interval.

Experimental realization of directed percolation criticality in turbulent liquid crystals.

This is a comprehensive report on the phase transition between two turbulent states of electroconvection in nematic liquid crystals, which was recently found by the authors to be in the directed percolation (DP) universality class [K. A. Takeuchi, Phys.

Directed percolation criticality in turbulent liquid crystals.

We experimentally investigate the critical behavior of a phase transition between two topologically different turbulent states of electrohydrodynamic convection in nematic liquid crystals. The statistical properties of the observed spatiotemporal intermittency regimes are carefully determined, yielding a complete set of static critical exponents in full agreement with those defining the directed percolation class in 2+1 dimensions. This constitutes the first clear and comprehensive experimental evidence of an absorbing phase transition in this prominent nonequilibrium universality class.