An attempt to construct the stroma of cornea using primary cultured corneal cells.

The number of patients currently awaiting corneal transplantation has resulted in the need to develop an artificial corneal replacement. In this study, we aimed to construct the corneal stroma using non-transformed corneal cells and a perfusion cell culture method. Corneal cells isolated from chicken embryos or rabbit and were embedded in the alkaline solubilized collagen gels crosslinked by TSG (Pentaerythritol polyethyleneglycol ether tetrasuccinimidyl glutarate).

Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium.

The cornea is a transparent tissue of the eye, which is responsible for the refraction of incoming light. Both biological corneal equivalents and synthetic keratoprostheses have been developed to replace donor tissue as a means to restore vision. However, both designs have drawbacks in terms of stability and biocompatibility.